Distribution of proteins in pulmonary edema. The value of fractional concentrations

C. L. Sprung, W. M. Long, E. H. Marcial, Roland Schein, R. E. Parker, T. Shomer, K. L. Brigham

Research output: Contribution to journalArticle

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Abstract

Pulmonary edema fluid (PEF) and serum (S) were obtained from 14 patients with cardiac pulmonary edema (CPE) and 25 noncardiac pulmonary edema (NCPE) patients. The type of pulmonary edema was based on a clinical classification. The protein content of PEF was not significantly different between CPE (3.89 ± 0.25 g/dl, mean ± SEM) and NCPE (4.35 ± 0.34 g/dl) patients, but the protein content of serum was different (7.18 ± 0.27 versus 5.13 ± 0.23, respectively, p < 0.001). As expected then, the PEF/S ratio was greater in NCPE than in CPE (0.85 ± 0.06 versus 0.54 ± 0.03, respectively, p < 0.05). Thus, differences in the PEF/S ratios in CPE as compared with those in NCPE are independent of mean edema fluid protein content and dependent on differences in serum protein content. PEF and S proteins were separated into 9 fractions of increasing molecular radius by electrophoresis, and fractional concentrations (percent of total protein content) were calculated. The fractional concentrations of these combined fractions of serum proteins were not different between CPE and NCPE. Fractional PEF/S ratios were significantly greater for combined fractions I-III (p < 0.01) in CPE than in NCPE and significantly less in fractions VII-IX (p < 0.01). In CPE, a higher percentage of proteins with smaller molecular radii (45 Å) enter the airway compartment in part because of higher hydrostatic pressures, whereas in NCPE, larger proteins enter the airways consequent to increased permeability to proteins with molecular radii greater than 72 Å. The permeability of proteins of intermediate size (45 to 72 Å) was not different between CPE and NCPE. Thus, analysis of fractional PEF/S is more useful than PEF/S protein ratios in identifying and differentiating pathophysiologic mechanisms of CPE and NCPE.

Original languageEnglish
Pages (from-to)957-963
Number of pages7
JournalAmerican Review of Respiratory Disease
Volume136
Issue number4
StatePublished - Dec 1 1987

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Pulmonary Edema
Cardiac Edema
Proteins
Blood Proteins
Protein S
Permeability

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Sprung, C. L., Long, W. M., Marcial, E. H., Schein, R., Parker, R. E., Shomer, T., & Brigham, K. L. (1987). Distribution of proteins in pulmonary edema. The value of fractional concentrations. American Review of Respiratory Disease, 136(4), 957-963.

Distribution of proteins in pulmonary edema. The value of fractional concentrations. / Sprung, C. L.; Long, W. M.; Marcial, E. H.; Schein, Roland; Parker, R. E.; Shomer, T.; Brigham, K. L.

In: American Review of Respiratory Disease, Vol. 136, No. 4, 01.12.1987, p. 957-963.

Research output: Contribution to journalArticle

Sprung, CL, Long, WM, Marcial, EH, Schein, R, Parker, RE, Shomer, T & Brigham, KL 1987, 'Distribution of proteins in pulmonary edema. The value of fractional concentrations', American Review of Respiratory Disease, vol. 136, no. 4, pp. 957-963.
Sprung CL, Long WM, Marcial EH, Schein R, Parker RE, Shomer T et al. Distribution of proteins in pulmonary edema. The value of fractional concentrations. American Review of Respiratory Disease. 1987 Dec 1;136(4):957-963.
Sprung, C. L. ; Long, W. M. ; Marcial, E. H. ; Schein, Roland ; Parker, R. E. ; Shomer, T. ; Brigham, K. L. / Distribution of proteins in pulmonary edema. The value of fractional concentrations. In: American Review of Respiratory Disease. 1987 ; Vol. 136, No. 4. pp. 957-963.
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abstract = "Pulmonary edema fluid (PEF) and serum (S) were obtained from 14 patients with cardiac pulmonary edema (CPE) and 25 noncardiac pulmonary edema (NCPE) patients. The type of pulmonary edema was based on a clinical classification. The protein content of PEF was not significantly different between CPE (3.89 ± 0.25 g/dl, mean ± SEM) and NCPE (4.35 ± 0.34 g/dl) patients, but the protein content of serum was different (7.18 ± 0.27 versus 5.13 ± 0.23, respectively, p < 0.001). As expected then, the PEF/S ratio was greater in NCPE than in CPE (0.85 ± 0.06 versus 0.54 ± 0.03, respectively, p < 0.05). Thus, differences in the PEF/S ratios in CPE as compared with those in NCPE are independent of mean edema fluid protein content and dependent on differences in serum protein content. PEF and S proteins were separated into 9 fractions of increasing molecular radius by electrophoresis, and fractional concentrations (percent of total protein content) were calculated. The fractional concentrations of these combined fractions of serum proteins were not different between CPE and NCPE. Fractional PEF/S ratios were significantly greater for combined fractions I-III (p < 0.01) in CPE than in NCPE and significantly less in fractions VII-IX (p < 0.01). In CPE, a higher percentage of proteins with smaller molecular radii (45 {\AA}) enter the airway compartment in part because of higher hydrostatic pressures, whereas in NCPE, larger proteins enter the airways consequent to increased permeability to proteins with molecular radii greater than 72 {\AA}. The permeability of proteins of intermediate size (45 to 72 {\AA}) was not different between CPE and NCPE. Thus, analysis of fractional PEF/S is more useful than PEF/S protein ratios in identifying and differentiating pathophysiologic mechanisms of CPE and NCPE.",
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