Distribution of nNOS positive neurons in dorsal root ganglia following nerve injury

D. Čížková, S. Jergová, M. Čížek, J. Maršala

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study we have observed the possible role of neuronal nitric oxide synthase (nNOS) in spinal sensitization after a different peripheral nerve injury accomplished by two surgical procedures: i) the complete sciatic nerve ligation (CSL) and ii) spared nerve injury (SNI). The nNOS immunoreactivity was monitored bilaterally in the (L4-L6) dorsal root ganglion cells (DRGs) in rats 14 days post surgery. Behavioral responses to mechanical and thermal non noxious stimuli were observed before and after surgery. In all animals with the CSL and SNI, an intense tactile and cold allodynia developed from day three and lasted up to 14 days on the ipsilateral hindpaw. Immunocytochemical staining revealed a significant increase of nNOS positive neurons in the ipsilateral DRGs (L4-L6) in the SNL rats (̃8.5-fold) and in the (CSL) (̃9.7-fold). However, no such changes were detected in the DRGs of contralateral side. Marked alterations of nNOS in corresponding DRGs may contribute to spinal sensory processing as well as to the development of neuronal plasticity in the superficial dorsal horn.

Original languageEnglish (US)
Pages (from-to)17-18
Number of pages2
JournalPsychiatrie
Volume5
Issue numberSUPPL. 2
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Psychiatry and Mental health

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