Distribution of intraportally implanted microspheres and fluorescent islets in mice

Erik Von Seth, Daniel Nyqvist, Arne Andersson, Per Ola Carlsson, Martin Köhler, Göran Mattsson, Astrid Nordin, Per Olof Berggren, Leif Jansson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The aim of the study was to evaluate the distribution of intraportally transplanted islets in mice. We initially administered 2000 polystyrene microspheres with a diameter of 50 μm intraportally into normoglycemic C57BL/6 mice. In separate experiments other mice were injected similarly with 300 microspheres each with a diameter of 100 or 200 μm. One week later the animals were killed, and the lungs and livers were removed and divided into lobes. The number of microspheres in each individual liver lobe and in the lungs was counted using a stereomicroscope. In other experiments, athymic C57BL/6 mice were similarly implanted with 250 islets isolated from transgenic mice expressing the enhanced yellow fluorescent protein in the islet cells. The distribution of microspheres and islets was independent of size, and fairly homogenous within the liver, with the exception of the caudate lobe, which contained fewer microspheres and islets, respectively. Approximately one third of all microspheres and islets were present as aggregates. Eighty-five to 90% of the implanted microspheres were identified in the liver sections, whereas 60-65% of the implanted islets were recovered. Aggregates or single fluorescent cells were observed in the liver of islet-implanted mice. We conclude that islets and microspheres implanted into the liver distribute fairly homogenously and quite a few of them exist as aggregates or, with respect to islets, as fragments.

Original languageEnglish (US)
Pages (from-to)621-627
Number of pages7
JournalCell Transplantation
Volume16
Issue number6
DOIs
StatePublished - Jan 1 2007

Keywords

  • Distribution
  • Engraftment
  • Liver
  • Microspheres
  • Pancreatic islets

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

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  • Cite this

    Von Seth, E., Nyqvist, D., Andersson, A., Carlsson, P. O., Köhler, M., Mattsson, G., Nordin, A., Berggren, P. O., & Jansson, L. (2007). Distribution of intraportally implanted microspheres and fluorescent islets in mice. Cell Transplantation, 16(6), 621-627. https://doi.org/10.3727/000000007783465055