Distribution of albumin in the normal monkey eye as revealed by Evans blue fluorescence microscopy

R. L. Radius, D. R. Anderson

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Since intravenously injected Evans blue binds irreversibly to serum albumin, its distribution reflects albumin exchange between the intravascular and extravascular tissue compartments. In histologic specimens examined by fluorescence microscopy, extravasated Evans blue-albumin complex was identified within the ciliary body and trabecular meshwork of normal monkey eyes. In eyes fixed by intra-arterial perfusion of fixative, no dye was identified in the choroid, retina, or optic nerve. With immersion fixation, however, some extravasation was seen in the choroid and adjacent optic nerve. In some specimens, the optic nerve was stained not only with material apparently leaking from the choroid but also from a breakdown of the blood-brain barrier in the major disc vasculature during the interval before fixative penetrates into the tissue. Perfusion fixation must be used to avoid this artifact, and freezing techniques would be even better.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - Dec 1 1980

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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