TY - JOUR
T1 - Distribution and Compartmentalization of Human Circulating and Tissue-Resident Memory T Cell Subsets
AU - Sathaliyawala, Taheri
AU - Kubota, Masaru
AU - Yudanin, Naomi
AU - Turner, Damian
AU - Camp, Philip
AU - Thome, Joseph J.C.
AU - Bickham, Kara L.
AU - Lerner, Harvey
AU - Goldstein, Michael
AU - Sykes, Megan
AU - Kato, Tomoaki
AU - Farber, Donna L.
N1 - Funding Information:
This work was supported by NIH “Challenge” grant RC1AI086164 awarded to D.L.F. We wish to gratefully acknowledge the generosity of the donor families and the outstanding efforts of the NYODN transplant coordinators and staff for making this study possible. We also wish to thank D. Corrigan and M. Yu for help with the tissue processing, S.-H. Ho for help with the flow cytometry, and S. Reiner for critical reading of this manuscript.
PY - 2013/1/24
Y1 - 2013/1/24
N2 - Knowledge of human T cells derives chiefly from studies of peripheral blood, whereas their distribution and function in tissues remains largely unknown. Here, we present a unique analysis of human T cells in lymphoid and mucosal tissues obtained from individual organ donors, revealing tissue-intrinsic compartmentalization of naive, effector, and memory subsets conserved between diverse individuals. Effector memory CD4+ T cells producing IL-2 predominated in mucosal tissues and accumulated as central memory subsets in lymphoid tissue, whereas CD8+ T cells were maintained as naive subsets in lymphoid tissues and IFN-γ-producing effector memory CD8+ T cells in mucosal sites. The T cell activation marker CD69 was constitutively expressed by memory T cells in all tissues, distinguishing them from circulating subsets, with mucosal memory T cells exhibiting additional distinct phenotypic and functional properties. Our results provide an assessment of human T cell compartmentalization as a new baseline for understanding human adaptive immunity.
AB - Knowledge of human T cells derives chiefly from studies of peripheral blood, whereas their distribution and function in tissues remains largely unknown. Here, we present a unique analysis of human T cells in lymphoid and mucosal tissues obtained from individual organ donors, revealing tissue-intrinsic compartmentalization of naive, effector, and memory subsets conserved between diverse individuals. Effector memory CD4+ T cells producing IL-2 predominated in mucosal tissues and accumulated as central memory subsets in lymphoid tissue, whereas CD8+ T cells were maintained as naive subsets in lymphoid tissues and IFN-γ-producing effector memory CD8+ T cells in mucosal sites. The T cell activation marker CD69 was constitutively expressed by memory T cells in all tissues, distinguishing them from circulating subsets, with mucosal memory T cells exhibiting additional distinct phenotypic and functional properties. Our results provide an assessment of human T cell compartmentalization as a new baseline for understanding human adaptive immunity.
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U2 - 10.1016/j.immuni.2012.09.020
DO - 10.1016/j.immuni.2012.09.020
M3 - Article
C2 - 23260195
AN - SCOPUS:84872772721
VL - 38
SP - 187
EP - 197
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 1
ER -