Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis

Régis Tournebize, Søren S.L. Andersen, Fulvia Verde, Marcel Dorée, Eric Karsenti, Anthony A. Hyman

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Abstract

Assembly of a mitotic spindle requires the accurate regulation of microtubule dynamics which is accomplished, at least in part, by phosphorylation-dephosphorylation reactions. Here we have investigated the role of serine-threonine phosphatases in the control of microtubule dynamics using specific inhibitors in Xenopus egg extracts. Type 2A phosphatases are required to maintain the short steady-state length of microtubules in mitosis by regulating the level of microtubule catastrophes, in part by controlling the the microtubule-destabilizing activity and phosphorylation of Op18/stathmin. Type 1 phosphatases are only required for control of microtubule dynamics during the transitions into and out of mitosis. Thus, although both type 2A and type 1 phosphatases are involved in the regulation of microtubule dynamics, they have distinct, non-overlapping roles.

Original languageEnglish (US)
Pages (from-to)5537-5549
Number of pages13
JournalEMBO Journal
Volume16
Issue number18
DOIs
StatePublished - Sep 15 1997

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Keywords

  • Microtubule dynamics
  • Mitosis
  • Opl8
  • Phosphatase
  • Spindle

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Tournebize, R., Andersen, S. S. L., Verde, F., Dorée, M., Karsenti, E., & Hyman, A. A. (1997). Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis. EMBO Journal, 16(18), 5537-5549. https://doi.org/10.1093/emboj/16.18.5537