Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis

Etienne Savary, Jean Philippe Hugnot, Yolaine Chassigneux, Cecile Travo, Christophe Duperray, Thomas R Van De Water, Azel Zine

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In mammals, the permanence of hearing loss is due mostly to the incapacity of the cochlea to replace lost mechano-receptor cells (i.e., hair cells [HCs]). The generation of new HCs from a renewable source of progenitors is a principal requirement for developing a cell therapy within this sensory organ. A subset of stem cells, termed side population (SP), has been identified in several tissues of mammals. The ATP-binding cassette transporter Abcg2/Bcrp1 contributes to the specification of the SP phenotype and is proposed as a universal marker for stem/progenitor cells. A defining character of these SP cells is a high efflux capacity for Hoechst dye. Here, we demonstrate that Abcg2 transporter is expressed with two other stem/progenitor cell markers (i.e., Nestin and Musashi1) in distinct and overlapping domains of the supporting cells within the postnatal cochlea. We have developed and describe a fluorescence-activated cell sorting (FACS) technique that enables the purification of a discrete subpopulation of SP-supporting cells from the early postnatal mouse cochlea based on their ability to exclude Hoechst dye. These FACS-isolated cells can divide and express markers of stem/progenitor cells such as Abcg2, a determinant of the SP phenotype, and Musashi1, a neural stem/progenitor cell marker. These markers can differentiate cells expressing markers of HCs and supporting cells in vitro. Our observation that these SP cells are capable of differentiating into HC-like cells implies a possible use for such cells (i.e., the replacement of lost auditory HCs within damaged cochlea).

Original languageEnglish
Pages (from-to)332-339
Number of pages8
JournalStem Cells
Volume25
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Cochlea
Stem Cells
Population
Side-Population Cells
Mammals
Flow Cytometry
Coloring Agents
Auditory Hair Cells
Phenotype
Nestin
ATP-Binding Cassette Transporters
Neural Stem Cells
Cell- and Tissue-Based Therapy
Hearing Loss
Hair

Keywords

  • Abcg2 transporter
  • Hair cells
  • Hearing loss
  • Side population
  • Side population-supporting cells
  • Stem/progenitor cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Savary, E., Hugnot, J. P., Chassigneux, Y., Travo, C., Duperray, C., Van De Water, T. R., & Zine, A. (2007). Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis. Stem Cells, 25(2), 332-339. https://doi.org/10.1634/stemcells.2006-0303

Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis. / Savary, Etienne; Hugnot, Jean Philippe; Chassigneux, Yolaine; Travo, Cecile; Duperray, Christophe; Van De Water, Thomas R; Zine, Azel.

In: Stem Cells, Vol. 25, No. 2, 01.02.2007, p. 332-339.

Research output: Contribution to journalArticle

Savary, E, Hugnot, JP, Chassigneux, Y, Travo, C, Duperray, C, Van De Water, TR & Zine, A 2007, 'Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis', Stem Cells, vol. 25, no. 2, pp. 332-339. https://doi.org/10.1634/stemcells.2006-0303
Savary, Etienne ; Hugnot, Jean Philippe ; Chassigneux, Yolaine ; Travo, Cecile ; Duperray, Christophe ; Van De Water, Thomas R ; Zine, Azel. / Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis. In: Stem Cells. 2007 ; Vol. 25, No. 2. pp. 332-339.
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