Distinct defensin profiles in Neisseria gonorrhoeae and Chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions

Edith Porter, Huixia Yang, Sujata Yavagal, Gloria C. Preza, Omar Murillo, Heriberto Lima, Sheila Greene, Laily Mahoozi, Marcia Klein-Patel, Gill Diamond, Sunita Gulati, Tomas Ganz, Peter A. Rice, Alison J. Quayle

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Defensins are key participants in mucosal innate defense. The varied antimicrobial activity and differential distribution of defensins at mucosal sites indicate that peptide repertoires are tailored to site-specific innate defense requirements. Nonetheless, few studies have investigated changes in peptide profiles and function after in vivo pathogen challenge. Here, we determined defensin profiles in urethral secretions of healthy men and men with Chlamydia trachomatis- and Neisseria gonorrhoeae-mediated urethritis by immunoblotting for the epithelial defensins HBD1, HBD2, and HD5 and the neutrophil defensins HNP1 to -3 (HNP1-3). HBD1 was not detectable in secretions, and HBD2 was only induced in a small proportion of the urethritis patients; however, HD5 and HNP1-3 were increased in C. trachomatis infection and significantly elevated in M gonorrhoeae infection. When HNP1-3 levels were low, HD5 appeared mostly as the propeptide; however, when HNP1-3 levels were > 10 μg/ml, HD5 was proteolytically processed, suggesting neutrophil proteases might contribute to HD5 processing. HD5 and HNP1-3 were bactericidal against C. trachomatis and N. gonorrhoeae, but HD5 activity was dependent upon N-terminal processing of the peptide. In vitro proteolysis of proHD5 by neutrophil proteases and analysis of urethral secretions by surface-enhanced laser desorption ionization substantiated that neutrophils contribute the key convertases for proHDS in the urethra during these infections. This contrasts with the small intestine, where Paneth cells secrete both proHD5 and its processing enzyme, trypsin. In conclusion, we describe a unique defensin expression repertoire in response to inflammatory sexually transmitted infections and a novel host defense mechanism wherein epithelial cells collaborate with neutrophils to establish an antimicrobial barrier during infection.

Original languageEnglish
Pages (from-to)4823-4833
Number of pages11
JournalInfection and Immunity
Volume73
Issue number8
DOIs
StatePublished - Aug 1 2005

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Defensins
Urethritis
Neisseria gonorrhoeae
Chlamydia trachomatis
Cell Communication
Neutrophils
Epithelial Cells
Peptides
Peptide Hydrolases
Infection
Paneth Cells
Chlamydia Infections
Gonorrhea
Urethra
Sexually Transmitted Diseases
Immunoblotting
Trypsin
Proteolysis
Small Intestine
Lasers

ASJC Scopus subject areas

  • Immunology

Cite this

Distinct defensin profiles in Neisseria gonorrhoeae and Chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions. / Porter, Edith; Yang, Huixia; Yavagal, Sujata; Preza, Gloria C.; Murillo, Omar; Lima, Heriberto; Greene, Sheila; Mahoozi, Laily; Klein-Patel, Marcia; Diamond, Gill; Gulati, Sunita; Ganz, Tomas; Rice, Peter A.; Quayle, Alison J.

In: Infection and Immunity, Vol. 73, No. 8, 01.08.2005, p. 4823-4833.

Research output: Contribution to journalArticle

Porter, E, Yang, H, Yavagal, S, Preza, GC, Murillo, O, Lima, H, Greene, S, Mahoozi, L, Klein-Patel, M, Diamond, G, Gulati, S, Ganz, T, Rice, PA & Quayle, AJ 2005, 'Distinct defensin profiles in Neisseria gonorrhoeae and Chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions', Infection and Immunity, vol. 73, no. 8, pp. 4823-4833. https://doi.org/10.1128/IAI.73.8.4823-4833.2005
Porter, Edith ; Yang, Huixia ; Yavagal, Sujata ; Preza, Gloria C. ; Murillo, Omar ; Lima, Heriberto ; Greene, Sheila ; Mahoozi, Laily ; Klein-Patel, Marcia ; Diamond, Gill ; Gulati, Sunita ; Ganz, Tomas ; Rice, Peter A. ; Quayle, Alison J. / Distinct defensin profiles in Neisseria gonorrhoeae and Chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions. In: Infection and Immunity. 2005 ; Vol. 73, No. 8. pp. 4823-4833.
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abstract = "Defensins are key participants in mucosal innate defense. The varied antimicrobial activity and differential distribution of defensins at mucosal sites indicate that peptide repertoires are tailored to site-specific innate defense requirements. Nonetheless, few studies have investigated changes in peptide profiles and function after in vivo pathogen challenge. Here, we determined defensin profiles in urethral secretions of healthy men and men with Chlamydia trachomatis- and Neisseria gonorrhoeae-mediated urethritis by immunoblotting for the epithelial defensins HBD1, HBD2, and HD5 and the neutrophil defensins HNP1 to -3 (HNP1-3). HBD1 was not detectable in secretions, and HBD2 was only induced in a small proportion of the urethritis patients; however, HD5 and HNP1-3 were increased in C. trachomatis infection and significantly elevated in M gonorrhoeae infection. When HNP1-3 levels were low, HD5 appeared mostly as the propeptide; however, when HNP1-3 levels were > 10 μg/ml, HD5 was proteolytically processed, suggesting neutrophil proteases might contribute to HD5 processing. HD5 and HNP1-3 were bactericidal against C. trachomatis and N. gonorrhoeae, but HD5 activity was dependent upon N-terminal processing of the peptide. In vitro proteolysis of proHD5 by neutrophil proteases and analysis of urethral secretions by surface-enhanced laser desorption ionization substantiated that neutrophils contribute the key convertases for proHDS in the urethra during these infections. This contrasts with the small intestine, where Paneth cells secrete both proHD5 and its processing enzyme, trypsin. In conclusion, we describe a unique defensin expression repertoire in response to inflammatory sexually transmitted infections and a novel host defense mechanism wherein epithelial cells collaborate with neutrophils to establish an antimicrobial barrier during infection.",
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AU - Murillo, Omar

AU - Lima, Heriberto

AU - Greene, Sheila

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AU - Klein-Patel, Marcia

AU - Diamond, Gill

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