Abstract
The sulfur-containing amino acid, methionine, has a role in the physiological environment because of its strong interactions with metals. To understand these interactions of metals with methionine, one needs reliable dissociation constants for the protonated methionine species (NH 3 +CH(CH 2CH 2SCH 3)COOH; H 2B +). The values of stoichiometric dissociation constants, pK i*, for protonated methionine species (H 2B +⇔H ++HB, K 1; HB⇔H ++B -, K 2) were determined from potentiometric measurements in NaCl solutions as a function of ionic strength, 0.25-6.0 mol (kg H 2O) -1 and temperature (5-45 °C). The results were extrapolated to pure water using the Pitzer equations to estimate the activity of H +, H 2B +, HB and B - as a function of ionic strength and temperature. The resulting thermodynamic values of K 1 and K 2 were fit to the equations (T/K): ln K 1=69.0013-3496.58/(T/K)-10.9153 ln (T/K); ln K 2=116.4162-10638.02/(T/K)-18.0553 ln (T/K) with standard errors of 0.003 and 0.033, respectively, for ln K 1* and ln K 2*. Pitzer interaction parameters (λ HB-Na and ζ HB-Na-Cl) for the neutral HB were determined from literature data. The Pitzer parameters (β H2BCl 0, β H2BCl 1 and C H2BCl φ) for the interactions of H 2B + with Cl - and Na + with and B 2- (β NaB 0, β NaB 1 and C NaB φ) were also determined. These coefficients can be used to make reasonable estimates of the activity coefficients of methionine species and the pK i* for the dissociation of methionine in physiological solutions, composed mostly of NaCl over a wide range of temperature and ionic strength.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-87 |
| Number of pages | 9 |
| Journal | Biophysical Chemistry |
| Volume | 105 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 1 2003 |
Fingerprint
Keywords
- Dissociation constants
- Methionine species
- NaCl
- Protonated
ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Biophysics
Cite this
Dissociation constants of protonated methionine species in NaCl media. / Sharma, Virender K.; Zinger, Anthony; Millero, Frank J; De Stefano, Concetta.
In: Biophysical Chemistry, Vol. 105, No. 1, 01.08.2003, p. 79-87.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissociation constants of protonated methionine species in NaCl media
AU - Sharma, Virender K.
AU - Zinger, Anthony
AU - Millero, Frank J
AU - De Stefano, Concetta
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The sulfur-containing amino acid, methionine, has a role in the physiological environment because of its strong interactions with metals. To understand these interactions of metals with methionine, one needs reliable dissociation constants for the protonated methionine species (NH 3 +CH(CH 2CH 2SCH 3)COOH; H 2B +). The values of stoichiometric dissociation constants, pK i*, for protonated methionine species (H 2B +⇔H ++HB, K 1; HB⇔H ++B -, K 2) were determined from potentiometric measurements in NaCl solutions as a function of ionic strength, 0.25-6.0 mol (kg H 2O) -1 and temperature (5-45 °C). The results were extrapolated to pure water using the Pitzer equations to estimate the activity of H +, H 2B +, HB and B - as a function of ionic strength and temperature. The resulting thermodynamic values of K 1 and K 2 were fit to the equations (T/K): ln K 1=69.0013-3496.58/(T/K)-10.9153 ln (T/K); ln K 2=116.4162-10638.02/(T/K)-18.0553 ln (T/K) with standard errors of 0.003 and 0.033, respectively, for ln K 1* and ln K 2*. Pitzer interaction parameters (λ HB-Na and ζ HB-Na-Cl) for the neutral HB were determined from literature data. The Pitzer parameters (β H2BCl 0, β H2BCl 1 and C H2BCl φ) for the interactions of H 2B + with Cl - and Na + with and B 2- (β NaB 0, β NaB 1 and C NaB φ) were also determined. These coefficients can be used to make reasonable estimates of the activity coefficients of methionine species and the pK i* for the dissociation of methionine in physiological solutions, composed mostly of NaCl over a wide range of temperature and ionic strength.
AB - The sulfur-containing amino acid, methionine, has a role in the physiological environment because of its strong interactions with metals. To understand these interactions of metals with methionine, one needs reliable dissociation constants for the protonated methionine species (NH 3 +CH(CH 2CH 2SCH 3)COOH; H 2B +). The values of stoichiometric dissociation constants, pK i*, for protonated methionine species (H 2B +⇔H ++HB, K 1; HB⇔H ++B -, K 2) were determined from potentiometric measurements in NaCl solutions as a function of ionic strength, 0.25-6.0 mol (kg H 2O) -1 and temperature (5-45 °C). The results were extrapolated to pure water using the Pitzer equations to estimate the activity of H +, H 2B +, HB and B - as a function of ionic strength and temperature. The resulting thermodynamic values of K 1 and K 2 were fit to the equations (T/K): ln K 1=69.0013-3496.58/(T/K)-10.9153 ln (T/K); ln K 2=116.4162-10638.02/(T/K)-18.0553 ln (T/K) with standard errors of 0.003 and 0.033, respectively, for ln K 1* and ln K 2*. Pitzer interaction parameters (λ HB-Na and ζ HB-Na-Cl) for the neutral HB were determined from literature data. The Pitzer parameters (β H2BCl 0, β H2BCl 1 and C H2BCl φ) for the interactions of H 2B + with Cl - and Na + with and B 2- (β NaB 0, β NaB 1 and C NaB φ) were also determined. These coefficients can be used to make reasonable estimates of the activity coefficients of methionine species and the pK i* for the dissociation of methionine in physiological solutions, composed mostly of NaCl over a wide range of temperature and ionic strength.
KW - Dissociation constants
KW - Methionine species
KW - NaCl
KW - Protonated
UR - http://www.scopus.com/inward/record.url?scp=0043161971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0043161971&partnerID=8YFLogxK
U2 - 10.1016/S0301-4622(03)00137-6
DO - 10.1016/S0301-4622(03)00137-6
M3 - Article
C2 - 12932581
AN - SCOPUS:0043161971
VL - 105
SP - 79
EP - 87
JO - Biophysical Chemistry
JF - Biophysical Chemistry
SN - 0301-4622
IS - 1
ER -