Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila

Susan L. McNabb, James Baker, Julie Agapite, Hermann Steller, Lynn M. Riddiford, James W. Truman

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

The neuropeptide eclosion hormone (EH) is a key regulator of insect ecdysis. We tested the role of the two EH-producing neurons in Drosophila by using an EH cell-specific enhancer to activate cell death genes reaper and head involution defective to ablate the EH cells. In the EH cell knockout flies, larval and adult ecdyses were disrupted, yet a third of the knockouts emerged as adults, demonstrating that EH has a significant but nonessential role in ecdysis. The EH cell knockouts had discrete behavioral deficits, including slow, uncoordinated eclosion and an insensitivity to ecdysis- triggering hormone. The knockouts lacked the lights-on eclosion response despite having a normal circadian eclosion rhythm. This study represents a novel approach to the dissection of neuropeptide regulation of a complex behavioral program.

Original languageEnglish (US)
Pages (from-to)813-823
Number of pages11
JournalNeuron
Volume19
Issue number4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Drosophila
Molting
Neurons
Neuropeptides
Circadian Rhythm
eclosion hormone
Diptera
Insects
Dissection
Cell Death
Head
Hormones
Light
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

McNabb, S. L., Baker, J., Agapite, J., Steller, H., Riddiford, L. M., & Truman, J. W. (1997). Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila. Neuron, 19(4), 813-823. https://doi.org/10.1016/S0896-6273(00)80963-0

Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila. / McNabb, Susan L.; Baker, James; Agapite, Julie; Steller, Hermann; Riddiford, Lynn M.; Truman, James W.

In: Neuron, Vol. 19, No. 4, 01.01.1997, p. 813-823.

Research output: Contribution to journalArticle

McNabb, SL, Baker, J, Agapite, J, Steller, H, Riddiford, LM & Truman, JW 1997, 'Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila', Neuron, vol. 19, no. 4, pp. 813-823. https://doi.org/10.1016/S0896-6273(00)80963-0
McNabb, Susan L. ; Baker, James ; Agapite, Julie ; Steller, Hermann ; Riddiford, Lynn M. ; Truman, James W. / Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila. In: Neuron. 1997 ; Vol. 19, No. 4. pp. 813-823.
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