Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila

Susan L. McNabb, James D. Baker, Julie Agapite, Hermann Steller, Lynn M. Riddiford, James W. Truman

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

The neuropeptide eclosion hormone (EH) is a key regulator of insect ecdysis. We tested the role of the two EH-producing neurons in Drosophila by using an EH cell-specific enhancer to activate cell death genes reaper and head involution defective to ablate the EH cells. In the EH cell knockout flies, larval and adult ecdyses were disrupted, yet a third of the knockouts emerged as adults, demonstrating that EH has a significant but nonessential role in ecdysis. The EH cell knockouts had discrete behavioral deficits, including slow, uncoordinated eclosion and an insensitivity to ecdysis- triggering hormone. The knockouts lacked the lights-on eclosion response despite having a normal circadian eclosion rhythm. This study represents a novel approach to the dissection of neuropeptide regulation of a complex behavioral program.

Original languageEnglish (US)
Pages (from-to)813-823
Number of pages11
JournalNeuron
Volume19
Issue number4
DOIs
StatePublished - Oct 1997
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Disruption of a behavioral sequence by targeted death of peptidergic neurons in Drosophila'. Together they form a unique fingerprint.

  • Cite this