TY - JOUR
T1 - Disrupted Schwann cell-axon interactions in peripheral nerves of mice with altered L1-integrin interactions
AU - Itoh, Kyoko
AU - Fushiki, Shinji
AU - Kamiguchi, Hiroyuki
AU - Arnold, Bernd
AU - Altevogt, Peter
AU - Lemmon, Vance
N1 - Funding Information:
The authors would like to thank Maryanne Pendergast, Midori Hitomi and Eli Weaver for technical assistance and Ina Wanner for critical comments on the manuscript. V. Lemmon was supported by grants from the National Institutes of Health (CHD-HD39884, EY-05285, and EY-11373) and RIKEN. B. Arnold was supported by a grant from Sonderforschungsbereich 405, and P. Altevogt by a grant from Scheel Stiftung (Bonn, Germany). V. Lemmon holds the Walter G. Ross Chair in Developmental Neuroscience at the University of Miami.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/9
Y1 - 2005/9
N2 - The cell adhesion molecule L1 is important for peripheral nerve development. Mice lacking the 6th Ig domain of L1 (L1-6D mice) lose L1 homophilic binding and RGD dependent L1-integrin binding [Itoh, K., Cheng, L., Kamei, Y., Fushiki, S., Kamiguchi, H., Gutwein, P., Stoeck, A., Arnold, B., Altevogt, P., Lemmon, V., 2004. Brain development in mice lacking L1-L1 homophilic adhesion. J. Cell Biol. 165, 145-154]. We examined the ultrastructure of sciatic nerves from L1-6D at postnatal day 7 and 8 weeks. Unmyelinated axons frequently detached at the edge of Schwann cells, and naked axons were observed. Myelin was thinner in L1-6D and abnormal, multiple axons wrapped in a single myelin sheath were routinely observed. Previous work has shown that L1 on axons interacts with a heterophilic binding partner on Schwann cells to facilitate normal peripheral nerve formation. Taken together, it is likely that L1 on axons binds integrins on Schwann cells, resulting in interactions between axons and Schwann cells that are essential for ensheathment and myelination.
AB - The cell adhesion molecule L1 is important for peripheral nerve development. Mice lacking the 6th Ig domain of L1 (L1-6D mice) lose L1 homophilic binding and RGD dependent L1-integrin binding [Itoh, K., Cheng, L., Kamei, Y., Fushiki, S., Kamiguchi, H., Gutwein, P., Stoeck, A., Arnold, B., Altevogt, P., Lemmon, V., 2004. Brain development in mice lacking L1-L1 homophilic adhesion. J. Cell Biol. 165, 145-154]. We examined the ultrastructure of sciatic nerves from L1-6D at postnatal day 7 and 8 weeks. Unmyelinated axons frequently detached at the edge of Schwann cells, and naked axons were observed. Myelin was thinner in L1-6D and abnormal, multiple axons wrapped in a single myelin sheath were routinely observed. Previous work has shown that L1 on axons interacts with a heterophilic binding partner on Schwann cells to facilitate normal peripheral nerve formation. Taken together, it is likely that L1 on axons binds integrins on Schwann cells, resulting in interactions between axons and Schwann cells that are essential for ensheathment and myelination.
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U2 - 10.1016/j.mcn.2005.06.006
DO - 10.1016/j.mcn.2005.06.006
M3 - Article
C2 - 16039871
AN - SCOPUS:23644445672
VL - 30
SP - 131
EP - 136
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
SN - 1044-7431
IS - 1
ER -