Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle

J. M. Lester, D. I. Silber, Walter G Bradley, M. H. Cohen, R. P. Hirsch, J. F. Brenner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.

Original languageEnglish
Pages (from-to)557-571
Number of pages15
JournalHistochemistry
Volume75
Issue number4
DOIs
StatePublished - Dec 1 1982
Externally publishedYes

Fingerprint

Myosins
myosin
dyes
Coloring Agents
Staining and Labeling
Muscles
muscles
muscular dystrophy
Specific Gravity
Duchenne Muscular Dystrophy
muscle tissues
sampling
adenosinetriphosphatase
Adenosine Triphosphatases
skeletal muscle
Skeletal Muscle
Population
staining

ASJC Scopus subject areas

  • Anatomy

Cite this

Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle. / Lester, J. M.; Silber, D. I.; Bradley, Walter G; Cohen, M. H.; Hirsch, R. P.; Brenner, J. F.

In: Histochemistry, Vol. 75, No. 4, 01.12.1982, p. 557-571.

Research output: Contribution to journalArticle

Lester, J. M. ; Silber, D. I. ; Bradley, Walter G ; Cohen, M. H. ; Hirsch, R. P. ; Brenner, J. F. / Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle. In: Histochemistry. 1982 ; Vol. 75, No. 4. pp. 557-571.
@article{8e29f54540ee4d18867b298e4d26adfc,
title = "Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle",
abstract = "Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.",
author = "Lester, {J. M.} and Silber, {D. I.} and Bradley, {Walter G} and Cohen, {M. H.} and Hirsch, {R. P.} and Brenner, {J. F.}",
year = "1982",
month = "12",
day = "1",
doi = "10.1007/BF00640606",
language = "English",
volume = "75",
pages = "557--571",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle

AU - Lester, J. M.

AU - Silber, D. I.

AU - Bradley, Walter G

AU - Cohen, M. H.

AU - Hirsch, R. P.

AU - Brenner, J. F.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.

AB - Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.

UR - http://www.scopus.com/inward/record.url?scp=0020403748&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020403748&partnerID=8YFLogxK

U2 - 10.1007/BF00640606

DO - 10.1007/BF00640606

M3 - Article

C2 - 6184341

AN - SCOPUS:0020403748

VL - 75

SP - 557

EP - 571

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 4

ER -