Directing and potentiating stem cell-mediated angiogenesis and tissue repair by cell surface e-selectin coating

Zhao-Jun Liu, Pirouz Daftarian, Letícia Kovalski, Bo Wang, Runxia Tian, Diego M. Castilla, Emre Dikici, Victor L Perez Quinones, Sapna K Deo, Sylvia Daunert, Omaida C Velazquez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Stem cell therapy has emerged as a promising approach for treatment of a number of diseases, including delayed and non-healing wounds. However, targeted systemic delivery of therapeutic cells to the dysfunctional tissues remains one formidable challenge. Herein, we present a targeted nanocarrier-mediated cell delivery method by coating the surface of the cell to be delivered with dendrimer nanocarriers modified with adhesion molecules. Infused nanocarrier-coated cells reach to destination via recognition and association with the counterpart adhesion molecules highly or selectively expressed on the activated endothelium in diseased tissues. Once anchored on the activated endothelium, nanocarriers-coated transporting cells undergo transendothelial migration, extravasation and homing to the targeted tissues to execute their therapeutic role. We now demonstrate feasibility, efficacy and safety of our targeted nanocarrier for delivery of bone marrow cells (BMC) to cutaneous wound tissues and grafted corneas and its advantages over conventional BMC transplantation in mouse models for wound healing and neovascularization. This versatile platform is suited for targeted systemic delivery of virtually any type of therapeutic cell.

Original languageEnglish (US)
Article numbere0154053
JournalPLoS One
Volume11
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Selectins
angiogenesis
tissue repair
Stem cells
coatings
stem cells
Repair
Stem Cells
Tissue
Coatings
therapeutics
Bone
Adhesion
bone marrow cells
cells
endothelium
Dendrimers
animal injuries
Molecules
Endothelium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Directing and potentiating stem cell-mediated angiogenesis and tissue repair by cell surface e-selectin coating. / Liu, Zhao-Jun; Daftarian, Pirouz; Kovalski, Letícia; Wang, Bo; Tian, Runxia; Castilla, Diego M.; Dikici, Emre; Perez Quinones, Victor L; Deo, Sapna K; Daunert, Sylvia; Velazquez, Omaida C.

In: PLoS One, Vol. 11, No. 4, e0154053, 01.04.2016.

Research output: Contribution to journalArticle

Liu, Zhao-Jun ; Daftarian, Pirouz ; Kovalski, Letícia ; Wang, Bo ; Tian, Runxia ; Castilla, Diego M. ; Dikici, Emre ; Perez Quinones, Victor L ; Deo, Sapna K ; Daunert, Sylvia ; Velazquez, Omaida C. / Directing and potentiating stem cell-mediated angiogenesis and tissue repair by cell surface e-selectin coating. In: PLoS One. 2016 ; Vol. 11, No. 4.
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AU - Castilla, Diego M.

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