Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes

Heather D. Hickman, Kazuyo Takeda, Cara N. Skon, Faith R. Murray, Scott E. Hensley, Joshua Loomis, Glen N Barber, Jack R. Bennink, Jonathan W. Yewdell

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the lymph node and infected cells residing just beneath the subcapsular sinus. Naive CD8+ T cells rapidly migrated to infected cells in the peripheral interfollicular region and then formed tight interactions with dendritic cells, leading to complete T cell activation. Thus, antigen presentation at the lymph node periphery, not at lymphocyte exit sites in deeper lymph node venules, as dogma dictates, has a dominant function in antiviral CD8+ T cell activation.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalNature Immunology
Volume9
Issue number2
DOIs
StatePublished - Feb 1 2008

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Antiviral Agents
Lymph Nodes
T-Lymphocytes
Lymphocytes
Vesicular Stomatitis
DNA Viruses
Venules
Vaccinia virus
RNA Viruses
Antigen Presentation
Adaptive Immunity
Virion
Dendritic Cells
Viruses
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Hickman, H. D., Takeda, K., Skon, C. N., Murray, F. R., Hensley, S. E., Loomis, J., ... Yewdell, J. W. (2008). Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes. Nature Immunology, 9(2), 155-165. https://doi.org/10.1038/ni1557

Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes. / Hickman, Heather D.; Takeda, Kazuyo; Skon, Cara N.; Murray, Faith R.; Hensley, Scott E.; Loomis, Joshua; Barber, Glen N; Bennink, Jack R.; Yewdell, Jonathan W.

In: Nature Immunology, Vol. 9, No. 2, 01.02.2008, p. 155-165.

Research output: Contribution to journalArticle

Hickman, HD, Takeda, K, Skon, CN, Murray, FR, Hensley, SE, Loomis, J, Barber, GN, Bennink, JR & Yewdell, JW 2008, 'Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes', Nature Immunology, vol. 9, no. 2, pp. 155-165. https://doi.org/10.1038/ni1557
Hickman HD, Takeda K, Skon CN, Murray FR, Hensley SE, Loomis J et al. Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes. Nature Immunology. 2008 Feb 1;9(2):155-165. https://doi.org/10.1038/ni1557
Hickman, Heather D. ; Takeda, Kazuyo ; Skon, Cara N. ; Murray, Faith R. ; Hensley, Scott E. ; Loomis, Joshua ; Barber, Glen N ; Bennink, Jack R. ; Yewdell, Jonathan W. / Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes. In: Nature Immunology. 2008 ; Vol. 9, No. 2. pp. 155-165.
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