Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes

Heather D. Hickman, Kazuyo Takeda, Cara N. Skon, Faith R. Murray, Scott E. Hensley, Joshua Loomis, Glen N. Barber, Jack R. Bennink, Jonathan W. Yewdell

Research output: Contribution to journalArticlepeer-review

193 Scopus citations


It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the lymph node and infected cells residing just beneath the subcapsular sinus. Naive CD8+ T cells rapidly migrated to infected cells in the peripheral interfollicular region and then formed tight interactions with dendritic cells, leading to complete T cell activation. Thus, antigen presentation at the lymph node periphery, not at lymphocyte exit sites in deeper lymph node venules, as dogma dictates, has a dominant function in antiviral CD8+ T cell activation.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalNature Immunology
Issue number2
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Direct priming of antiviral CD8<sup>+</sup> T cells in the peripheral interfollicular region of lymph nodes'. Together they form a unique fingerprint.

Cite this