Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis

George I. Malinin, Francis J. Hornicek, Theodore I. Malinin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0°C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalBioscience reports
Volume10
Issue number4
DOIs
StatePublished - Aug 1 1990

Keywords

  • blastogenesis
  • chromic acid
  • lymphocytes
  • membrane oxidation
  • periodic acid
  • sialic acid

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis'. Together they form a unique fingerprint.

  • Cite this