Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis

George I. Malinin; Francis J. Hornicek; Theodore I. Malinin

doi:10.1007/BF01117234

Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis

George I. Malinin, Francis J. Hornicek, Theodore I. Malinin

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Abstract

Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO₃ at 0°C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H₅IO₆) and thereafter by 1.0 mM CrO₃, the viability and [³H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO₃. The reversal of the oxidation sequence restored [³H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO₃ with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [³H]-TdR incorporation, but it does not induce blastogenesis.

Original language	English (US)
Pages (from-to)	347-352
Number of pages	6
Journal	Bioscience reports
Volume	10
Issue number	4
DOIs	https://doi.org/10.1007/BF01117234
State	Published - Aug 1 1990

Keywords

blastogenesis
chromic acid
lymphocytes
membrane oxidation
periodic acid
sialic acid

ASJC Scopus subject areas

Cell Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01117234

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title = "Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis",

abstract = "Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0°C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.",

keywords = "blastogenesis, chromic acid, lymphocytes, membrane oxidation, periodic acid, sialic acid",

author = "Malinin, {George I.} and Hornicek, {Francis J.} and Malinin, {Theodore I.}",

year = "1990",

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doi = "10.1007/BF01117234",

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TY - JOUR

T1 - Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis

AU - Malinin, George I.

AU - Hornicek, Francis J.

AU - Malinin, Theodore I.

PY - 1990/8/1

Y1 - 1990/8/1

N2 - Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0°C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.

AB - Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0°C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.

KW - blastogenesis

KW - chromic acid

KW - lymphocytes

KW - membrane oxidation

KW - periodic acid

KW - sialic acid

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ER -

Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis

Abstract

Keywords

ASJC Scopus subject areas

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