Direct measurements of increased free cytoplasmic Ca2+ in mouse pancreatic β-cells following stimulation by hypoglycemie sulfonylureas

Hakan Abrahamsson, Per Olof Berggren, Patrik Rorsman

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The effects of the hypoglycemic sulfonylureas tolbutamide and glibenclamide on free cytoplasmic Ca2+, [Ca2+]i, were compared with that of a depolarizing concentration of K+ in dispered and cultured pancreatic β-cells from ob/ob mice. [Ca2+]i was measured with the fluorescent Ca2+-indicator quin2. The basal level corresponded to 150 nM and increased to 600 nM after exposure to 30.9 mM K+. The corresponding levels after stimulation with 1 μM glibenclamide and 100 μM tolbutamide were 390 and 270 nM respectively. K+ depolarization increased [Ca2+]i more rapidly than either of the sulfonylureas. It is suggested that the increased [Ca2+]i obtained after stimulation by sulfonylureas is due to depolarization of the β-cells with subsequent entry of Ca2+ through voltage-dependent channels.

Original languageEnglish (US)
Pages (from-to)21-24
Number of pages4
JournalFEBS letters
Volume190
Issue number1
DOIs
StatePublished - Oct 7 1985

Keywords

  • Cytoplasmic Ca
  • Hypoglycemic sulfonylurea
  • Pancreatic β-cell
  • Quin2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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