Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects

Krishna V Komanduri, Sean M. Donahoe, Walter J. Moretto, Diane K. Schmidt, Geraldine Gillespie, Graham S. Ogg, Mario Roederer, Douglas F. Nixon, Joseph M. McCune

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Data from murine models of chronic viral infection suggest that CD4+ T-cell responses to viral pathogens are important in sustaining the number and/or function of CD8+ cytotoxic T-cell (CTL) effectors. In this study, we used cytokine flow cytometry (CFC), staining with HLA-A*0201-peptide tetramers, and peptide stimulation with epitopic peptides to study functional CD4+ and CD8+ T-cell responses to cytomegalovirus (CMV) in human subjects coinfected with CMV and the human immunodeficiency virus, type 1 (HIV-1). We show that strong CD4+ and CD8+ T-cell responses to CMV antigens are sustained over time in HIV-1-infected individuals. Those who maintain a strong CD4+ T-cell response to CMV are also likely to maintain higher frequencies of CD8+ T cells capable of binding to HLA-A*0201-CMV pp65 (A2-pp65) tetramers as well as responses to pp65 peptide stimulation with effector cytokine production. These data support the hypothesis that declines in frequencies of CD4+ T-cell responses to CMV are associated with an inability to sustain high levels of CMV-specific CDS+ T-cell responses in HIV-1-infected subjects. These declines may precede the onset of CMV-associated end organ disease.

Original languageEnglish
Pages (from-to)459-470
Number of pages12
JournalVirology
Volume279
Issue number2
DOIs
StatePublished - Jan 15 2001
Externally publishedYes

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Cytomegalovirus
HIV-1
T-Lymphocytes
Peptides
Cytokines
Virus Diseases
Flow Cytometry
Staining and Labeling
Antigens

Keywords

  • AIDS
  • FACS
  • HIV
  • Human
  • Infectious immunity virus
  • T lymphocytes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Komanduri, K. V., Donahoe, S. M., Moretto, W. J., Schmidt, D. K., Gillespie, G., Ogg, G. S., ... McCune, J. M. (2001). Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. Virology, 279(2), 459-470. https://doi.org/10.1006/viro.2000.0697

Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. / Komanduri, Krishna V; Donahoe, Sean M.; Moretto, Walter J.; Schmidt, Diane K.; Gillespie, Geraldine; Ogg, Graham S.; Roederer, Mario; Nixon, Douglas F.; McCune, Joseph M.

In: Virology, Vol. 279, No. 2, 15.01.2001, p. 459-470.

Research output: Contribution to journalArticle

Komanduri, KV, Donahoe, SM, Moretto, WJ, Schmidt, DK, Gillespie, G, Ogg, GS, Roederer, M, Nixon, DF & McCune, JM 2001, 'Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects', Virology, vol. 279, no. 2, pp. 459-470. https://doi.org/10.1006/viro.2000.0697
Komanduri KV, Donahoe SM, Moretto WJ, Schmidt DK, Gillespie G, Ogg GS et al. Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. Virology. 2001 Jan 15;279(2):459-470. https://doi.org/10.1006/viro.2000.0697
Komanduri, Krishna V ; Donahoe, Sean M. ; Moretto, Walter J. ; Schmidt, Diane K. ; Gillespie, Geraldine ; Ogg, Graham S. ; Roederer, Mario ; Nixon, Douglas F. ; McCune, Joseph M. / Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. In: Virology. 2001 ; Vol. 279, No. 2. pp. 459-470.
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