Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects

Krishna V. Komanduri, Sean M. Donahoe, Walter J. Moretto, Diane K. Schmidt, Geraldine Gillespie, Graham S. Ogg, Mario Roederer, Douglas F. Nixon, Joseph M. McCune

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Data from murine models of chronic viral infection suggest that CD4+ T-cell responses to viral pathogens are important in sustaining the number and/or function of CD8+ cytotoxic T-cell (CTL) effectors. In this study, we used cytokine flow cytometry (CFC), staining with HLA-A*0201-peptide tetramers, and peptide stimulation with epitopic peptides to study functional CD4+ and CD8+ T-cell responses to cytomegalovirus (CMV) in human subjects coinfected with CMV and the human immunodeficiency virus, type 1 (HIV-1). We show that strong CD4+ and CD8+ T-cell responses to CMV antigens are sustained over time in HIV-1-infected individuals. Those who maintain a strong CD4+ T-cell response to CMV are also likely to maintain higher frequencies of CD8+ T cells capable of binding to HLA-A*0201-CMV pp65 (A2-pp65) tetramers as well as responses to pp65 peptide stimulation with effector cytokine production. These data support the hypothesis that declines in frequencies of CD4+ T-cell responses to CMV are associated with an inability to sustain high levels of CMV-specific CDS+ T-cell responses in HIV-1-infected subjects. These declines may precede the onset of CMV-associated end organ disease.

Original languageEnglish (US)
Pages (from-to)459-470
Number of pages12
JournalVirology
Volume279
Issue number2
DOIs
StatePublished - Jan 15 2001
Externally publishedYes

Keywords

  • AIDS
  • FACS
  • HIV
  • Human
  • Infectious immunity virus
  • T lymphocytes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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    Komanduri, K. V., Donahoe, S. M., Moretto, W. J., Schmidt, D. K., Gillespie, G., Ogg, G. S., Roederer, M., Nixon, D. F., & McCune, J. M. (2001). Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. Virology, 279(2), 459-470. https://doi.org/10.1006/viro.2000.0697