Direct evidence for the formation of a monolayer from a bilayer. An ellipsometric study at the nitrogen-water interface.

C. Salesse, D. Ducharme, Roger Leblanc

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Direct evidence for the formation of a monolayer from a bilayer was measured by ellipsometry after spreading unilamellar vesicles of dioleoyl phosphatidylcholine (DOPC) at the nitrogen-water interface. The ellipsometric isotherms of DOPC vesicles and DOPC spread from an organic solvent were compared and found similar. From the observed ellipsometric angle (delta delta) in the plateau region (-1.04 degrees) and literature data for refractive indices of an anisotropic film similar to DOPC, we have calculated a thickness of 20 +/- 1 A. These results strongly suggest that, similarly to DOPC spread from an organic solvent, DOPC vesicles form a monolayer when spread at the nitrogen-water interface.

Original languageEnglish
Pages (from-to)351-352
Number of pages2
JournalBiophysical Journal
Volume52
Issue number2
StatePublished - Aug 1 1987
Externally publishedYes

Fingerprint

Nitrogen
Water
Unilamellar Liposomes
Refractometry
1,2-oleoylphosphatidylcholine

ASJC Scopus subject areas

  • Biophysics

Cite this

Direct evidence for the formation of a monolayer from a bilayer. An ellipsometric study at the nitrogen-water interface. / Salesse, C.; Ducharme, D.; Leblanc, Roger.

In: Biophysical Journal, Vol. 52, No. 2, 01.08.1987, p. 351-352.

Research output: Contribution to journalArticle

@article{7946b662176d4a5eac8756cae705da9c,
title = "Direct evidence for the formation of a monolayer from a bilayer. An ellipsometric study at the nitrogen-water interface.",
abstract = "Direct evidence for the formation of a monolayer from a bilayer was measured by ellipsometry after spreading unilamellar vesicles of dioleoyl phosphatidylcholine (DOPC) at the nitrogen-water interface. The ellipsometric isotherms of DOPC vesicles and DOPC spread from an organic solvent were compared and found similar. From the observed ellipsometric angle (delta delta) in the plateau region (-1.04 degrees) and literature data for refractive indices of an anisotropic film similar to DOPC, we have calculated a thickness of 20 +/- 1 A. These results strongly suggest that, similarly to DOPC spread from an organic solvent, DOPC vesicles form a monolayer when spread at the nitrogen-water interface.",
author = "C. Salesse and D. Ducharme and Roger Leblanc",
year = "1987",
month = "8",
day = "1",
language = "English",
volume = "52",
pages = "351--352",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "2",

}

TY - JOUR

T1 - Direct evidence for the formation of a monolayer from a bilayer. An ellipsometric study at the nitrogen-water interface.

AU - Salesse, C.

AU - Ducharme, D.

AU - Leblanc, Roger

PY - 1987/8/1

Y1 - 1987/8/1

N2 - Direct evidence for the formation of a monolayer from a bilayer was measured by ellipsometry after spreading unilamellar vesicles of dioleoyl phosphatidylcholine (DOPC) at the nitrogen-water interface. The ellipsometric isotherms of DOPC vesicles and DOPC spread from an organic solvent were compared and found similar. From the observed ellipsometric angle (delta delta) in the plateau region (-1.04 degrees) and literature data for refractive indices of an anisotropic film similar to DOPC, we have calculated a thickness of 20 +/- 1 A. These results strongly suggest that, similarly to DOPC spread from an organic solvent, DOPC vesicles form a monolayer when spread at the nitrogen-water interface.

AB - Direct evidence for the formation of a monolayer from a bilayer was measured by ellipsometry after spreading unilamellar vesicles of dioleoyl phosphatidylcholine (DOPC) at the nitrogen-water interface. The ellipsometric isotherms of DOPC vesicles and DOPC spread from an organic solvent were compared and found similar. From the observed ellipsometric angle (delta delta) in the plateau region (-1.04 degrees) and literature data for refractive indices of an anisotropic film similar to DOPC, we have calculated a thickness of 20 +/- 1 A. These results strongly suggest that, similarly to DOPC spread from an organic solvent, DOPC vesicles form a monolayer when spread at the nitrogen-water interface.

UR - http://www.scopus.com/inward/record.url?scp=0023391539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023391539&partnerID=8YFLogxK

M3 - Article

VL - 52

SP - 351

EP - 352

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 2

ER -