Direct evidence for opposite effects of D-glucose and D-glyceraldehyde on cytoplasmic pH of mouse pancreatic β-cells

Per Arkhammar, Per Olof Berggren, Patrik Rorsman

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The effects of D-glucose, D-glyceraldehyde, glibenclamide, D-600, NH4+ and high concentrations of K+ on cytoplasmic pH (pHi) were investigated in dispersed and cultured pancreatic β-cells from ob/ob mice. The cytoplasmic pH was measured with the fluorescent H+-indicator quene 1. The average pHi value in resting β-cells was 6.71. Addition of 20 m M of the physiological stimulus D-glucose increased pHi with 0.05 units. Both glibenclamide and high concentrations of K+ decreased pHi. The latter effects were completely reversed by D-600, supporting the notion that free cytoplasmic Ca2+ can be involved in the regulation of pHi. In contrast to D-glucose, 10m M of D-glyceraldehyde decreased pHi by 0.09 units, an effect persisting even in the presence of D-600. From the present study it is evident that D-glyceraldehyde and D-glucose have opposite effects on pHi in pancreatic β-cells.

Original languageEnglish (US)
Pages (from-to)355-361
Number of pages7
JournalBioscience reports
Volume6
Issue number4
DOIs
StatePublished - Apr 1 1986

Keywords

  • D-glucose
  • D-glyceraldehyde
  • pancreatic B cells
  • pH (cytoplasmic)

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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