Direct evidence for acute and massive norepinephrine release in the hippocampus during transient ischemia

M. Y T Globus, R. Busto, W. Dalton Dietrich, E. Martinez, I. Valdes, Myron Ginsberg

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Recent studies suggest that norepinephrine (NE) may play a regulatory role in neuronal cell death in the hippocampus after transient ischemia. However, ischemia-induced changes in extracellular NE release have not been demonstrated. In the present study, we utilized the microdialysis technique to measure extracellular NE levels in the hippocampus before, during, and after 20 min of global ischemia induced by two-vessel occlusion combined with systemic hypotension in the rat. Stable basal concentrations of extracellular NE were detected in three consecutive samples collected prior to ischemia (1.86 ± 1.21 pmol/ml of perfusate mean ± SEM). During ischemia, NE levels increased to 30.1 ± 5.5 pmol/ml, representing an 18-fold increase. The levels gradually returned to baseline by 40 min of reperfusion. These results are the first to demonstrate that acute and massive extracellular release of NE occurs in the hippocampus during ischemia and early recirculation. These results support the hypothesis that the activation of the noradrenergic system may play a significant role in modulating the development of ischemic neuronal damage.

Original languageEnglish
Pages (from-to)892-896
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume9
Issue number6
StatePublished - Jan 1 1989

Fingerprint

Hippocampus
Norepinephrine
Ischemia
Microdialysis
Hypotension
Reperfusion
Cell Death

Keywords

  • global cerebral ischemia
  • hippocampus
  • microdialysis
  • norepinephrine

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

Cite this

Direct evidence for acute and massive norepinephrine release in the hippocampus during transient ischemia. / Globus, M. Y T; Busto, R.; Dalton Dietrich, W.; Martinez, E.; Valdes, I.; Ginsberg, Myron.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 9, No. 6, 01.01.1989, p. 892-896.

Research output: Contribution to journalArticle

@article{9268868da9f74725a8b455973e5f2b77,
title = "Direct evidence for acute and massive norepinephrine release in the hippocampus during transient ischemia",
abstract = "Recent studies suggest that norepinephrine (NE) may play a regulatory role in neuronal cell death in the hippocampus after transient ischemia. However, ischemia-induced changes in extracellular NE release have not been demonstrated. In the present study, we utilized the microdialysis technique to measure extracellular NE levels in the hippocampus before, during, and after 20 min of global ischemia induced by two-vessel occlusion combined with systemic hypotension in the rat. Stable basal concentrations of extracellular NE were detected in three consecutive samples collected prior to ischemia (1.86 ± 1.21 pmol/ml of perfusate mean ± SEM). During ischemia, NE levels increased to 30.1 ± 5.5 pmol/ml, representing an 18-fold increase. The levels gradually returned to baseline by 40 min of reperfusion. These results are the first to demonstrate that acute and massive extracellular release of NE occurs in the hippocampus during ischemia and early recirculation. These results support the hypothesis that the activation of the noradrenergic system may play a significant role in modulating the development of ischemic neuronal damage.",
keywords = "global cerebral ischemia, hippocampus, microdialysis, norepinephrine",
author = "Globus, {M. Y T} and R. Busto and {Dalton Dietrich}, W. and E. Martinez and I. Valdes and Myron Ginsberg",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "9",
pages = "892--896",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Direct evidence for acute and massive norepinephrine release in the hippocampus during transient ischemia

AU - Globus, M. Y T

AU - Busto, R.

AU - Dalton Dietrich, W.

AU - Martinez, E.

AU - Valdes, I.

AU - Ginsberg, Myron

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Recent studies suggest that norepinephrine (NE) may play a regulatory role in neuronal cell death in the hippocampus after transient ischemia. However, ischemia-induced changes in extracellular NE release have not been demonstrated. In the present study, we utilized the microdialysis technique to measure extracellular NE levels in the hippocampus before, during, and after 20 min of global ischemia induced by two-vessel occlusion combined with systemic hypotension in the rat. Stable basal concentrations of extracellular NE were detected in three consecutive samples collected prior to ischemia (1.86 ± 1.21 pmol/ml of perfusate mean ± SEM). During ischemia, NE levels increased to 30.1 ± 5.5 pmol/ml, representing an 18-fold increase. The levels gradually returned to baseline by 40 min of reperfusion. These results are the first to demonstrate that acute and massive extracellular release of NE occurs in the hippocampus during ischemia and early recirculation. These results support the hypothesis that the activation of the noradrenergic system may play a significant role in modulating the development of ischemic neuronal damage.

AB - Recent studies suggest that norepinephrine (NE) may play a regulatory role in neuronal cell death in the hippocampus after transient ischemia. However, ischemia-induced changes in extracellular NE release have not been demonstrated. In the present study, we utilized the microdialysis technique to measure extracellular NE levels in the hippocampus before, during, and after 20 min of global ischemia induced by two-vessel occlusion combined with systemic hypotension in the rat. Stable basal concentrations of extracellular NE were detected in three consecutive samples collected prior to ischemia (1.86 ± 1.21 pmol/ml of perfusate mean ± SEM). During ischemia, NE levels increased to 30.1 ± 5.5 pmol/ml, representing an 18-fold increase. The levels gradually returned to baseline by 40 min of reperfusion. These results are the first to demonstrate that acute and massive extracellular release of NE occurs in the hippocampus during ischemia and early recirculation. These results support the hypothesis that the activation of the noradrenergic system may play a significant role in modulating the development of ischemic neuronal damage.

KW - global cerebral ischemia

KW - hippocampus

KW - microdialysis

KW - norepinephrine

UR - http://www.scopus.com/inward/record.url?scp=0024377189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024377189&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 892

EP - 896

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 6

ER -