Direct determination of manganese in microgram amounts of pancreatic tissue by electrothermal atomic absorption spectrometry

Patrik Rorsman, Per Olof Berggren

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Manganese is determined by the direct insertion of freeze-dried biological samples (1-15 μg) or by injection of 2-μl samples of perifusion medium into the graphite furnace. At the most sensitive wavelength (279.5 nm), down to 0.2 pmol of manganese can be measured in the perifusion medium as well as the endogenous manganese in the endocrine and exocrine parts of the pancreas. The latter values were 0.08 ± 0.01 and 0.16 ± 0.01 mmol Mn kg-1 (dry wt.), respectively. A less sensitive wavelength (403.1 nm) is employed for measuring the larger amounts obtained after incubating the specimens in the presence of manganese(II).

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalAnalytica Chimica Acta
Volume140
Issue number1
DOIs
StatePublished - Aug 1 1982
Externally publishedYes

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Atomic absorption spectrometry
Manganese
spectrometry
manganese
Spectrum Analysis
Tissue
wavelength
Exocrine Pancreas
Wavelength
Graphite
graphite
Furnaces
Injections
tissue

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Direct determination of manganese in microgram amounts of pancreatic tissue by electrothermal atomic absorption spectrometry. / Rorsman, Patrik; Berggren, Per Olof.

In: Analytica Chimica Acta, Vol. 140, No. 1, 01.08.1982, p. 325-329.

Research output: Contribution to journalArticle

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