Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors

K. Saidas Nair, Susan M. Hanson, Matthew J. Kennedy, James B. Hurley, Vsevolod V. Gurevich, Vladlen Z Slepak

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Proper function of visual arrestin is indispensable for rapid signal shut-off in rod photoreceptors. Dramatic light-dependent changes in its subcellular localization are believed to play an important role in light adaptation of photoreceptor cells. Here we show that visual arrestin binds microtubules. The truncated splice variant of visual arrestin, p44, demonstrates dramatically higher affinity for microtubules than the full-length protein (p48). Enhanced microtubule binding of p44 underlies its earlier reported preferential localization to detergent-resistant membranes, where it is anchored via membrane-associated microtubules in a rhodopsin-independent fashion. Experiments with purified proteins demonstrate that arrestin interaction with microtubules is direct and does not require any additional protein partners. Most importantly, arrestin interactions with microtubules and light-activated phosphorylated rhodopsin are mutually exclusive, suggesting that microtubule interaction may play a role in keeping p44 arrestin away from rhodopsin in dark-adapted photoreceptors.

Original languageEnglish
Pages (from-to)41240-41248
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number39
DOIs
StatePublished - Sep 24 2004

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Arrestin
Retinal Rod Photoreceptor Cells
Microtubules
Rhodopsin
Membranes
Proteins
Ocular Adaptation
Light
Photoreceptor Cells
Detergents

ASJC Scopus subject areas

  • Biochemistry

Cite this

Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. / Nair, K. Saidas; Hanson, Susan M.; Kennedy, Matthew J.; Hurley, James B.; Gurevich, Vsevolod V.; Slepak, Vladlen Z.

In: Journal of Biological Chemistry, Vol. 279, No. 39, 24.09.2004, p. 41240-41248.

Research output: Contribution to journalArticle

Nair, K. Saidas ; Hanson, Susan M. ; Kennedy, Matthew J. ; Hurley, James B. ; Gurevich, Vsevolod V. ; Slepak, Vladlen Z. / Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 39. pp. 41240-41248.
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