Dimethylaminoethanol (deanol) metabolism in rat brain and its effect on acetylcholine synthesis

R. S. Jope, D. J. Jenden

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Specific methods utilizing combined gas chromatography mass spectrometry were used to measure the metabolism of [2H6]deanol and its effects on acetylcholine concentration in vitro and in vivo. In vitro [2H6]deanol was rapidly taken up by rat brain synaptosomes, but was neither methylated nor acetylated. [2H6]Deanol was a weak competitive inhibitor of the high affinity transport of [2H4]choline, thus reducing the synthesis of [2H4]acetylcholine. In vivo [2H6]deanol was present in the brain after i.p. or p.o. administration, but was not methylated or acetylated. Treatment of rats with [2H6]deanol significantly increased the concentration of choline in the plasma and brain but did not alter the concentration of acetylcholine in the brain. Treatment of rats with atropine (to stimulate acetylcholine turnover) or with hemicholinium-3 (to inhibit the high affinity transport of choline) did not reveal any effect of [2H6]deanol on acetylcholine synthesis in vivo. However, since [2H6]deanol did increase brain choline, it may prove therapeutically useful when the production of choline is reduced or when the utilization of choline for the synthesis of acetylcholine is impaired.

Original languageEnglish (US)
Pages (from-to)472-479
Number of pages8
JournalJournal of Pharmacology and Experimental Therapeutics
Volume211
Issue number3
StatePublished - Dec 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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