Dimer monomer transition and dimer re-formation play important role for ATM cellular function during DNA repair

Fengxia Du, Minjie Zhang, Xiaohua Li, Caiyun Yang, Hao Meng, Dong Wang, Shuang Chang, Ye Xu, Brendan Price, Yingli Sun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The ATM protein kinase, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks, mediates responses to ionizing radiation in mammalian cells. Here we show that ATM is held inactive in unirradiated cells as a dimer and phosphorylates the opposite strand of the dimer in response to DNA damage. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM cannot phosphorylate the substrates when it could not undergo dimer monomer transition. After DNA repair, the active monomer will undergo dephosphorylation to form dimer again and dephosphorylation is critical for dimer re-formation. Our work reveals novel function of ATM dimer monomer transition and explains why ATM dimer monomer transition plays such important role for ATM cellular activity during DNA repair.

Original languageEnglish (US)
Pages (from-to)1034-1039
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume452
Issue number4
DOIs
StatePublished - Oct 3 2014
Externally publishedYes

Fingerprint

Automatic teller machines
DNA Repair
Dimers
Repair
Monomers
Ataxia Telangiectasia Mutated Proteins
Double-Stranded DNA Breaks
Protein-Serine-Threonine Kinases
DNA
Ionizing Radiation
Protein Kinases
Serine
DNA Damage
Phosphotransferases
Ionizing radiation
Cells
Irradiation
Substrates

Keywords

  • ATM activation
  • Dephosphorylation
  • DNA repair
  • Intermolecular phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Dimer monomer transition and dimer re-formation play important role for ATM cellular function during DNA repair. / Du, Fengxia; Zhang, Minjie; Li, Xiaohua; Yang, Caiyun; Meng, Hao; Wang, Dong; Chang, Shuang; Xu, Ye; Price, Brendan; Sun, Yingli.

In: Biochemical and Biophysical Research Communications, Vol. 452, No. 4, 03.10.2014, p. 1034-1039.

Research output: Contribution to journalArticle

Du, Fengxia ; Zhang, Minjie ; Li, Xiaohua ; Yang, Caiyun ; Meng, Hao ; Wang, Dong ; Chang, Shuang ; Xu, Ye ; Price, Brendan ; Sun, Yingli. / Dimer monomer transition and dimer re-formation play important role for ATM cellular function during DNA repair. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 452, No. 4. pp. 1034-1039.
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AU - Wang, Dong

AU - Chang, Shuang

AU - Xu, Ye

AU - Price, Brendan

AU - Sun, Yingli

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