Digestion of the "proteolytic resistant" f-subclass of rabbit secretory IgA. Localization of f-subclass allotypic specificities to the Fc fragments

Thomas Malek, Katherine L. Knight

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Previous studies have established differential susceptibility of the rabbit sIgA subclass to proteolytic digestion; the sIgA-f sublcalss molecules are resistant and the sIgA-g subclass molecules are susceptible to proteolytic digestion. In this investigation rabbit sIgA was reduced and alkylated, digested with papain and subsequently subjected to gel filtration on Sephadex G-200. Antigenic analysis, by a quantitative radio-precipitation assay, and physio-chemical analysis revealed that one fraction from gel filtration contained an Fc fragment of sIgA-f subclass molecules; this Fc fragment also contained some determinants of the f-allotypic specificities. These studies indicate that the differential susceptibility of the rabbit sIgA subclass to proteolytic digestion may be related to differences in intercahin disulfice bonds.

Original languageEnglish
Pages (from-to)493-496
Number of pages4
JournalImmunochemistry
Volume14
Issue number7
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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Secretory Immunoglobulin A
Digestion
Rabbits
Gel Chromatography
Papain
Radio

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Digestion of the "proteolytic resistant" f-subclass of rabbit secretory IgA. Localization of f-subclass allotypic specificities to the Fc fragments. / Malek, Thomas; Knight, Katherine L.

In: Immunochemistry, Vol. 14, No. 7, 01.01.1977, p. 493-496.

Research output: Contribution to journalArticle

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