Digestion of the "proteolytic resistant" f-subclass of rabbit secretory IgA. Localization of f-subclass allotypic specificities to the Fc fragments

Thomas R. Malek, Katherine L. Knight

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Abstract

Previous studies have established differential susceptibility of the rabbit sIgA subclass to proteolytic digestion; the sIgA-f sublcalss molecules are resistant and the sIgA-g subclass molecules are susceptible to proteolytic digestion. In this investigation rabbit sIgA was reduced and alkylated, digested with papain and subsequently subjected to gel filtration on Sephadex G-200. Antigenic analysis, by a quantitative radio-precipitation assay, and physio-chemical analysis revealed that one fraction from gel filtration contained an Fc fragment of sIgA-f subclass molecules; this Fc fragment also contained some determinants of the f-allotypic specificities. These studies indicate that the differential susceptibility of the rabbit sIgA subclass to proteolytic digestion may be related to differences in intercahin disulfice bonds.

Original languageEnglish (US)
Pages (from-to)493-496
Number of pages4
JournalImmunochemistry
Volume14
Issue number7
DOIs
StatePublished - Jul 1977
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

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