Diffuse optical monitoring of repeated cerebral ischemia in mice

Yu Shang, Lei Chen, Michal J Toborek, Guoqiang Yu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Occlusions of bilateral common carotid arteries (bi-CCA) in mice are popular models for the investigation of transient forebrain ischemia. Currently available technologies for assessing cerebral blood flow (CBF) and oxygenation in ischemic mice have limitations. This study tests a novel near-infrared diffuse correlation spectroscopy (DCS) flow-oximeter for monitoring both CBF and cerebral oxygenation in mice undergoing repeated transient forebrain ischemia. Concurrent flow measurements in a mouse brain were first conducted for validation purposes; DCS measurement was found highly correlated with laser Doppler measurement (R2 = 0.94) and less susceptible to motion artifacts. With unique designs in experimental protocols and fiber-optic probes, we have demonstrated high sensitivities of DCS flow-oximeter in detecting the regional heterogeneity of CBF responses in different hemispheres and global changes of both CBF and cerebral oxygenation across two hemispheres in mice undergoing repeated 2-minute bi-CCA occlusions over 5 days. More than 75% CBF reductions were found during bi-CCA occlusions in mice, which may be considered as a threshold to determine a successful bi-CCA occlusion. With the progress of repeated 2-minute bi-CCA occlusions over days, a longitudinal decline in the magnitudes of CBF reduction was observed, indicating the brain adaptation to cerebral ischemia through the repeated preconditioning.

Original languageEnglish
Pages (from-to)20301-20315
Number of pages15
JournalOptics Express
Volume19
Issue number21
DOIs
StatePublished - Oct 10 2011
Externally publishedYes

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ischemia
blood flow
occlusion
mice
arteries
oxygenation
hemispheres
brain
spectroscopy
preconditioning
flow measurement
artifacts
fiber optics
thresholds
probes
sensitivity
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Diffuse optical monitoring of repeated cerebral ischemia in mice. / Shang, Yu; Chen, Lei; Toborek, Michal J; Yu, Guoqiang.

In: Optics Express, Vol. 19, No. 21, 10.10.2011, p. 20301-20315.

Research output: Contribution to journalArticle

Shang, Yu ; Chen, Lei ; Toborek, Michal J ; Yu, Guoqiang. / Diffuse optical monitoring of repeated cerebral ischemia in mice. In: Optics Express. 2011 ; Vol. 19, No. 21. pp. 20301-20315.
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