Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing

Garrett M. Goss, Nirupa Chaudhari, Joshua Hare, Raphael Nwojo, Barbara Seidler, Dieter Saur, Bradley J Goldstein

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Olfactory tissue undergoes lifelong renewal, due to the presence of basal neural stem cells. Multiple categories of globose basal stem cells have been identified, expressing markers such as Lgr5, Ascl1, GBC-2, and c-Kit. The differentiation potential of individual globose cells has remained unclear. Here, we utilized Cre/loxP lineage tracing with a multicolor reporter system to define c-Kit+ cell contributions at clonal resolution. We determined that reporter expression permitted identification of c-Kit derived progeny with fine cellular detail, and that clones were found to be comprised by neurons only, microvillar cells only, microvillar cells and neurons, or gland/duct cells. Quantification of reporter-labeled cells indicated that c-Kit+ cells behave as transit amplifying or immediate precursors, although we also found evidence for longer-term c-Kit+ cell contributions. Our results from the application of multicolor fate mapping delineate the clonal contributions of c-Kit+ cells to olfactory epithelial renewal, and provide novel insight into tissue maintenance of an adult neuroepithelium.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalDevelopmental Neurobiology
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2016

Keywords

  • Neurogenesis
  • Olfaction
  • Receptor tyrosine kinase
  • Regeneration
  • Stem cells

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

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