Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls: Metabolite analysis in glaucoma

Ciara Myer, Jordan Perez, Leila Abdelrahman, Roberto Mendez, Ram B. Khattri, Anna K. Junk, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticle

Abstract

We report an analysis of the aqueous humor (AH) metabolome of primary open angle glaucoma (POAG) in comparison to normal controls. The AH samples were obtained from human donors [control (n = 35), POAG (n = 23)]. The AH samples were subjected to one-dimensional 1H nuclear magnetic resonance (NMR) analyses on a Bruker Avance 600 MHz instrument with a 1.7 mM NMR probe. The same samples were then subjected to isotopic ratio outlier analysis (IROA) using a Q Exactive orbitrap mass spectrometer after chromatography on an Accela 600 HPLC. Clusterfinder Build 3.1.10 was used for identification and quantification based on long-term metabolite matrix standards. In total, 278 metabolites were identified in control samples and 273 in POAG AH. The metabolites identified were fed into previously reported proteome and genome information and the OmicsNet interaction network generator to construct a protein-metabolite interactions network with an embedded protein-protein network. Significant differences in metabolite composition in POAG compared to controls were identified indicating potential protein/gene pathways associated with these metabolites. These results will expand our previous understanding of the impeded AH metabolite composition, provide new insight into the regulation of AH outflow, and likely aid in future AH and trabecular meshwork multi-omics network analyses.

Original languageEnglish (US)
Article number108024
JournalExperimental Eye Research
Volume194
DOIs
StatePublished - May 2020

Keywords

  • Aqueous humor
  • Glaucoma
  • IROA
  • Metabolomics
  • NMR
  • POAG

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls: Metabolite analysis in glaucoma'. Together they form a unique fingerprint.

  • Cite this