Differentiation of an immortalized CNS neuronal cell line decreases their susceptibility to cytotoxic T cell lysis in vitro

Linda A. White, Robert W. Keane, Scott R. Whittemore

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

RN33B cells are temperature-sensitive neuronal cell line derived from rat E12 medullary raphe nucleus (Whittemore and White (1993) Brain Research 615, 27-40). Undifferentiated RN33B cells express class I but not class II antigens of the major histocompatibility complex (MHC), and intercellular adhesion molecule-1 (ICAM-1), a ligand for lymphocyte function associated antigen-1 (LFA-1), expressed on cytotoxic T lymphocytes (CTLs). Treatment of undifferentiated RN33B cells with interferon-γ (IFN-γ) upregulated both class I MHC and ICAM-1. After neuronal differentiated, expression of class I MHC antigens or ICAM-1 was undetected, even after IFN-γ treatment. The neuronally differentiated RN33B cells were also markedly less susceptible to to lysis by alloantigen-specific CTLs. These data suggest that intrinsic to the differentiation of CNS neurons is a mechanism to escape CTL-mediated cell lysis.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalJournal of Neuroimmunology
Volume49
Issue number1-2
DOIs
StatePublished - Jan 1994

Keywords

  • Class I MHC antigens
  • Cytotoxic T lymphocytes
  • Differentiation
  • ICAM-1
  • Neuronal cell line

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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