Differentiation and transformation of neural plate cells

Robert Keane, Leah A. Lipsich, Joan S. Brugge

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The developmental potential of presumptive neural plate cells of prestreak chick embryos (stage 1) and neural plate cells from definitive streak chick embryos (stage 4) has been examined in cell culture using specific markers that identify the major cell types in the vertebrate central nervous system. The prestreak presumptive neural plate (PSPNP) cells, stage 1, assume an epithelial appearance in vitro and synthesize cellular fibronectin, but do not express markers for the neuronal, astrocytic, melanocytic, or oligodendrocytic lineages. Conversely, definitive-streak neural plate (DSNP) cells contain precursors which express cell-type-specific markers for terminally differentiated neurons, astrocytes, and melanocytes, and synthesize an extracellular matrix of cellular fibronectin. Differentiation of DSNP cells in vitro can be prevented by infection with a temperature-sensitive mutant of Rous sarcoma virus (RSV), tsNY68. Differentiation of DSNP cell transformants can be resumed by a temperature shift to the nonpermissive temperature. The morphological and biochemical changes associated with tsNY68 transformation are accompanied by alterations in pp60src kinase activity in the transformed cells.

Original languageEnglish
Pages (from-to)38-52
Number of pages15
JournalDevelopmental Biology
Volume103
Issue number1
DOIs
StatePublished - Jan 1 1984

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Neural Plate
Chick Embryo
Fibronectins
Temperature
Rous sarcoma virus
Melanocytes
Astrocytes
Extracellular Matrix
Vertebrates
Phosphotransferases
Central Nervous System
Cell Culture Techniques
Neurons

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Differentiation and transformation of neural plate cells. / Keane, Robert; Lipsich, Leah A.; Brugge, Joan S.

In: Developmental Biology, Vol. 103, No. 1, 01.01.1984, p. 38-52.

Research output: Contribution to journalArticle

Keane, Robert ; Lipsich, Leah A. ; Brugge, Joan S. / Differentiation and transformation of neural plate cells. In: Developmental Biology. 1984 ; Vol. 103, No. 1. pp. 38-52.
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