Differential time course of effects of κ-opioid agonist treatment on dynorphin A levels and κ-opioid receptor density

Claudio D'Addario; Manuela Di Benedetto; Sari Izenwasser; Sanzio Candeletti; Patrizia Romualdi

doi:10.1385/JMN:24:2:307

Differential time course of effects of κ-opioid agonist treatment on dynorphin A levels and κ-opioid receptor density

Claudio D'Addario, Manuela Di Benedetto, Sari Izenwasser, Sanzio Candeletti, Patrizia Romualdi

Psychiatry & Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The effects of κ-opioid agonist treatment on κ-opioid receptor density and on dynorphin A levels in the rat brain were studied. Rats were treated with the selective κ-opioid agonist U-69593 or vehicle for 5 d. Dynorphin A levels and κ-opioid receptor binding were measured on day 8 (3 d after the last injection) or 22 (17 d after the last injection). On day 8, κ-opioid receptor density was increased in the hypothalamus of rats treated with U-69593; there were no changes in the frontal cortex or caudate putamen. In contrast, there was an increase in dynorphin A levels in the frontal cortex and no changes in hypothalamus and caudate putamen in response to U-69593. On day 22, B_max was decreased in frontal cortex and caudate putamen of U-69593-treated rats, whereas dynorphin A levels were increased in the caudate putamen and in the frontal cortex. These findings suggest that κ-opioid receptor agonist treatment has long-term, continually changing effects on the κ-opioid system.

Original language	English (US)
Pages (from-to)	307-314
Number of pages	8
Journal	Journal of Molecular Neuroscience
Volume	24
Issue number	2
DOIs	https://doi.org/10.1385/JMN:24:2:307
State	Published - Sep 2004

Keywords

κ-receptor
Dynorphin A
Opioids
Rat
U-69593

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry
Genetics

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title = "Differential time course of effects of κ-opioid agonist treatment on dynorphin A levels and κ-opioid receptor density",

abstract = "The effects of κ-opioid agonist treatment on κ-opioid receptor density and on dynorphin A levels in the rat brain were studied. Rats were treated with the selective κ-opioid agonist U-69593 or vehicle for 5 d. Dynorphin A levels and κ-opioid receptor binding were measured on day 8 (3 d after the last injection) or 22 (17 d after the last injection). On day 8, κ-opioid receptor density was increased in the hypothalamus of rats treated with U-69593; there were no changes in the frontal cortex or caudate putamen. In contrast, there was an increase in dynorphin A levels in the frontal cortex and no changes in hypothalamus and caudate putamen in response to U-69593. On day 22, Bmax was decreased in frontal cortex and caudate putamen of U-69593-treated rats, whereas dynorphin A levels were increased in the caudate putamen and in the frontal cortex. These findings suggest that κ-opioid receptor agonist treatment has long-term, continually changing effects on the κ-opioid system.",

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author = "Claudio D'Addario and {Di Benedetto}, Manuela and Sari Izenwasser and Sanzio Candeletti and Patrizia Romualdi",

note = "Funding Information: This work was supported by a grant from the Italian Ministry for the University and Scientific Research (MIUR ex60%) and by a grant from the National Institute on Drug Abuse/National Institutes of Health (DA-11960–SI). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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T1 - Differential time course of effects of κ-opioid agonist treatment on dynorphin A levels and κ-opioid receptor density

AU - D'Addario, Claudio

AU - Di Benedetto, Manuela

AU - Izenwasser, Sari

AU - Candeletti, Sanzio

AU - Romualdi, Patrizia

N1 - Funding Information: This work was supported by a grant from the Italian Ministry for the University and Scientific Research (MIUR ex60%) and by a grant from the National Institute on Drug Abuse/National Institutes of Health (DA-11960–SI). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/9

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N2 - The effects of κ-opioid agonist treatment on κ-opioid receptor density and on dynorphin A levels in the rat brain were studied. Rats were treated with the selective κ-opioid agonist U-69593 or vehicle for 5 d. Dynorphin A levels and κ-opioid receptor binding were measured on day 8 (3 d after the last injection) or 22 (17 d after the last injection). On day 8, κ-opioid receptor density was increased in the hypothalamus of rats treated with U-69593; there were no changes in the frontal cortex or caudate putamen. In contrast, there was an increase in dynorphin A levels in the frontal cortex and no changes in hypothalamus and caudate putamen in response to U-69593. On day 22, Bmax was decreased in frontal cortex and caudate putamen of U-69593-treated rats, whereas dynorphin A levels were increased in the caudate putamen and in the frontal cortex. These findings suggest that κ-opioid receptor agonist treatment has long-term, continually changing effects on the κ-opioid system.

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Differential time course of effects of κ-opioid agonist treatment on dynorphin A levels and κ-opioid receptor density

Abstract

Keywords

ASJC Scopus subject areas

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