Differential T-cell activation by B7-1 expression

Wei Li, Anthony Rosenzweig, Brigitte T. Huber

Research output: Contribution to journalArticle

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Abstract

T-cell receptor-mediated T-cell activation requires cosimulation signal, which can be provided by B7-1 molecule. Our previous study demonstrated that the coexpression of a covalent peptide/major histocompatibility complex class II molecule complex and costimulatory molecule B7-1 by recombinant adenovirus leads to synergy in peptide-specific T-cell activation. However, the viral antigen-specific T-cell activation is not enhanced by B7-1 expressed by the adenovirus. To verify the differential T cell activation by B7-1 and investigate its underlying mechanisms, we constructed an adenovirus coexpressing a covalent complex of hen egg lysozyme peptide/I-Ak (HEL46-61/I-Ak) and B7-1 in the present study. In vivo studies revealed that HEL46-61-specific T-cell response, but not viral antigen-specific T-cell response, was enhanced by B7-1 expression mediated by the adenovirus, suggesting that exogenous B7-1 expression may regulate T-cell response to these two different antigens through distinct mechanisms. Furthermore, our results revealed that antigen-presenting cells were unsusceptible to adenovirus infection in vivo. Based on these findings, the possible mechanism of differential B7-1 costimulation on peptide-specific and viral antigen-specific T-cell activation is discussed.

Original languageEnglish
Pages (from-to)336-342
Number of pages7
JournalImmunology
Volume109
Issue number3
DOIs
StatePublished - Jul 1 2003

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T-Lymphocytes
Adenoviridae
Viral Antigens
Peptides
CD80 Antigens
B7 Antigens
Peptide T
Adenoviridae Infections
Antigen-Presenting Cells
T-Cell Antigen Receptor
Major Histocompatibility Complex
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Differential T-cell activation by B7-1 expression. / Li, Wei; Rosenzweig, Anthony; Huber, Brigitte T.

In: Immunology, Vol. 109, No. 3, 01.07.2003, p. 336-342.

Research output: Contribution to journalArticle

Li, Wei ; Rosenzweig, Anthony ; Huber, Brigitte T. / Differential T-cell activation by B7-1 expression. In: Immunology. 2003 ; Vol. 109, No. 3. pp. 336-342.
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