Differential regulation of human T lymphoblast functions by IL-2 and IL-15

Silvia Bulfone-Paus, Horst Dürkop, Ralf Paus, Hans Krause, Thomas Pohl, Onu Adrian

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Interleukin 15 (IL-15) shares many functional properties with interleukin 2 (IL-2), although both cytokines probably also exert distinct functions. In order to screen for functional differences between IL-2 and IL-15 with respect to the control of T cell functions, we have stimulated human T lymphoblasts (hTBl) with IL-2 and/or IL-15 and have assessed the resulting changes in the following parameters: T cell proliferation; expression of various relevant surface markers; cytokine and receptor (α-chain) transcription; and IL-2 and IL-15 activity. Both cytokines equally upregulate standard activation markers such as CD25 and CD95 and downregulate CD27. However, IL-2 upregulates CD30, TNF receptor type II and CD40L expression significantly stronger than IL-15. IL-15 potentiates Con A-induced IL-2 secretion. Even though hTBl transcribe the IL-15 gene, they do not secrete IL-15 activity. These observations suggest that both cytokines can differentially regulate T cells, e.g. T cell functions relevant to the control of cell cycle progression and apoptosis, and/or that they can stimulate different T cell subsets. Moreover, IL-15 may potentiate IL-2-driven T cell responses.

Original languageEnglish (US)
Pages (from-to)507-513
Number of pages7
JournalCytokine
Volume9
Issue number7
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

    Fingerprint

Keywords

  • Human T lymphoblasts
  • IL-15
  • IL-2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Bulfone-Paus, S., Dürkop, H., Paus, R., Krause, H., Pohl, T., & Adrian, O. (1997). Differential regulation of human T lymphoblast functions by IL-2 and IL-15. Cytokine, 9(7), 507-513. https://doi.org/10.1006/cyto.1996.0194