Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens

E. W. Thompson, R. Reich, T. B. Shima, A. Albini, J. Graf, G. R. Martin, R. B. Dickson, Marc E Lippman

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Estrogen increases the ability of the estrogen-dependent MCF-7 human breast cancer cell line to both proliferate and invade through an artificial basement membrane. In studying the response of MCF-7 cells to various antiestrogens, we found that 4-hydroxytamoxifen and tamoxifen inhibited cell proliferation but increased their invasiveness. In contrast, the structurally unrelated benzothiophene antiestrogens, LY117018 and LY156758, were potent antiproliferative agents which did not stimulate invasiveness. The differential effects of these antiestrogenic agents on invasion correlated with changes in production of collagenase IV, while no significant change was seen in the chemotactic activity of the cells. Invasiveness was increased by 17β-estradiol or 4-hydroxytamoxifen after a few hours of treatment and was rapidly lost when 17β-estradiol was withdrawn. Stimulation of invasiveness with 17β-estradiol was blocked by the antiestrogen, LY117018. Cells from the MDA-MB-231 line which lacks estrogen receptors were not affected by estrogen or antiestrogen in terms of proliferation or invasion. These studies indicate that the invasiveness of MCF-7 cells is regulated by antiestrogens through the estrogen receptor and may be mediated by collagenase IV activity. Antiestrogens which reduce both the proliferation and invasiveness of these cells may be interesting new candidates for clinical application.

Original languageEnglish
Pages (from-to)6764-6768
Number of pages5
JournalCancer Research
Volume48
Issue number23
StatePublished - Dec 1 1988
Externally publishedYes

Fingerprint

Estrogen Receptor Modulators
Breast Neoplasms
LY 117018
Growth
Estradiol
Estrogens
MCF-7 Cells
Collagenases
Estrogen Receptors
Cell Proliferation
Artificial Membranes
Tamoxifen
Basement Membrane
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Thompson, E. W., Reich, R., Shima, T. B., Albini, A., Graf, J., Martin, G. R., ... Lippman, M. E. (1988). Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens. Cancer Research, 48(23), 6764-6768.

Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens. / Thompson, E. W.; Reich, R.; Shima, T. B.; Albini, A.; Graf, J.; Martin, G. R.; Dickson, R. B.; Lippman, Marc E.

In: Cancer Research, Vol. 48, No. 23, 01.12.1988, p. 6764-6768.

Research output: Contribution to journalArticle

Thompson, EW, Reich, R, Shima, TB, Albini, A, Graf, J, Martin, GR, Dickson, RB & Lippman, ME 1988, 'Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens', Cancer Research, vol. 48, no. 23, pp. 6764-6768.
Thompson EW, Reich R, Shima TB, Albini A, Graf J, Martin GR et al. Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens. Cancer Research. 1988 Dec 1;48(23):6764-6768.
Thompson, E. W. ; Reich, R. ; Shima, T. B. ; Albini, A. ; Graf, J. ; Martin, G. R. ; Dickson, R. B. ; Lippman, Marc E. / Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens. In: Cancer Research. 1988 ; Vol. 48, No. 23. pp. 6764-6768.
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