Differential regulation of brain opioid receptors following repeated cocaine administration to guinea pigs

Yossef Itzhak

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent studies implied a possible involvement of the opioid system in the central nervous system (CNS) effects of cocaine. The present study was undertaken to determine specific alterations in opioid receptor binding following repeated exposure of guinea pigs to cocaine. Animals were treated with either saline or cocaine (40 mg/kg per day; i.p.) for 7 days, sacrificed 24 h after the treatment, and various brain regions were assayed for [3H]DAMGO, [3H]DPDPE and [3H]U-69,593 binding to the three major types of opioid receptors: mu, delta and kappa, respectively. A significant down-regulation of mu-opioid receptors (65-70% of control Bmax) was detected in the frontal cortex, amygdala, thalamus and hippocampus of cocaine-treated animals. Other brain regions examined did not endure changes in mu-opioid receptor binding. In contrast, no significant change in delta-opioid receptor binding was detected at any of the brain regions investigated. Alteration in kappa-opioid receptor binding following exposure to cocaine was found only in the cerebellum: an up-regulation (136% of control Bmax) was observed. These findings indicate that repeated administration of cocaine induces differential regulation of opioid receptors. The down-regulation of mu-opioid receptors in discrete limbic regions may be associated with the addicting properties of cocaine.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalDrug and Alcohol Dependence
Volume33
Issue number1
DOIs
StatePublished - Jan 1 1993

Fingerprint

Guinea
Opioid Receptors
Cocaine
Brain
brain
Guinea Pigs
mu Opioid Receptor
regulation
animal
kappa Opioid Receptor
delta Opioid Receptor
Animals
Down-Regulation
D-Penicillamine (2,5)-Enkephalin
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Neurology
Frontal Lobe
Amygdala
Thalamus
Cerebellum

Keywords

  • cocaine
  • limbic brain regions
  • opioid receptors
  • receptor regulation

ASJC Scopus subject areas

  • Medicine(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

Cite this

Differential regulation of brain opioid receptors following repeated cocaine administration to guinea pigs. / Itzhak, Yossef.

In: Drug and Alcohol Dependence, Vol. 33, No. 1, 01.01.1993, p. 53-59.

Research output: Contribution to journalArticle

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