Differential regulation of σ and PCP receptors after chronic administration of haloperidol and phencyclidine in mice

Yossef Itzhak, S. Alerhand

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The existence of multiple receptor sites for the psychotomimetic agents phencyclidine (PCP) and some opiate-benzomorphans such as (+)N-allylnormetazocine ([+]SKF 10,047) in the mammalian central nervous system is well documented. These are: 1) σ/PCP (σp) site, which binds both PCP and psychotomimetic opiates but not antipsychotics such as haloperidol, 2) PCP site, which selectively binds PCP analogs, and 3) σ/haloperidol (σh) site, for which certain antipsychotics and (+)SKF 10,047, but not PCP analogs, display high affinity. In this study we examined the regulation of these receptor sites after chronic treatment of mice with either PCP or haloperidol. The following radiolabele ligands were used to assess binding to the various receptor subtypes: [3H]-1-[1-[3-hydroxyphenyl)cyclohexyl]piperidine ([3H]PCP-3-OH; σp and PCP sites), [3H]thienyl-phencyclidine ([3H]TCP; PCP site), (+)-[3H]SKF 10,047 (σp and σh sites), and [3H]haloperidol (σh and D-2 dopamine receptors). Treatment of mice for 1, 7, 14, and 21 days with PCP (10 mg·kg-1·day-1) failed to induce variations in σp, σh, and PCP receptor binding. However, similar treatment with haloperidol (4 mg·kg-1·day-1) induced: 1) complete elimination of the binding to σh sites, 2) up-regulation of D-2 dopamine receptors, and 3) no change in σp and PCP receptor binding after 14 or 21 days of treatment. However, a single day of haloperidol treatment or in vitro incubation of mouse brain membranes with haloperidol failed to alter receptor binding. This study suggests that prolonged treatment of mice with haloperidol induces a loss in σh receptors that are presumably associated with certain psychotomimetic effects. This phenomenon is accompanied by an up-regulation of D-2 dopamine receptors.

Original languageEnglish
Pages (from-to)1868-1872
Number of pages5
JournalFASEB Journal
Volume3
Issue number7
StatePublished - Jan 1 1989

Fingerprint

Phencyclidine Receptors
haloperidol
Hallucinogens
Phencyclidine
Haloperidol
receptors
mice
Opiate Alkaloids
Dopamine Receptors
piperidines
Antipsychotic Agents
Benzomorphans
Up-Regulation
central nervous system
Neurology
brain

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Differential regulation of σ and PCP receptors after chronic administration of haloperidol and phencyclidine in mice. / Itzhak, Yossef; Alerhand, S.

In: FASEB Journal, Vol. 3, No. 7, 01.01.1989, p. 1868-1872.

Research output: Contribution to journalArticle

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abstract = "The existence of multiple receptor sites for the psychotomimetic agents phencyclidine (PCP) and some opiate-benzomorphans such as (+)N-allylnormetazocine ([+]SKF 10,047) in the mammalian central nervous system is well documented. These are: 1) σ/PCP (σp) site, which binds both PCP and psychotomimetic opiates but not antipsychotics such as haloperidol, 2) PCP site, which selectively binds PCP analogs, and 3) σ/haloperidol (σh) site, for which certain antipsychotics and (+)SKF 10,047, but not PCP analogs, display high affinity. In this study we examined the regulation of these receptor sites after chronic treatment of mice with either PCP or haloperidol. The following radiolabele ligands were used to assess binding to the various receptor subtypes: [3H]-1-[1-[3-hydroxyphenyl)cyclohexyl]piperidine ([3H]PCP-3-OH; σp and PCP sites), [3H]thienyl-phencyclidine ([3H]TCP; PCP site), (+)-[3H]SKF 10,047 (σp and σh sites), and [3H]haloperidol (σh and D-2 dopamine receptors). Treatment of mice for 1, 7, 14, and 21 days with PCP (10 mg·kg-1·day-1) failed to induce variations in σp, σh, and PCP receptor binding. However, similar treatment with haloperidol (4 mg·kg-1·day-1) induced: 1) complete elimination of the binding to σh sites, 2) up-regulation of D-2 dopamine receptors, and 3) no change in σp and PCP receptor binding after 14 or 21 days of treatment. However, a single day of haloperidol treatment or in vitro incubation of mouse brain membranes with haloperidol failed to alter receptor binding. This study suggests that prolonged treatment of mice with haloperidol induces a loss in σh receptors that are presumably associated with certain psychotomimetic effects. This phenomenon is accompanied by an up-regulation of D-2 dopamine receptors.",
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N2 - The existence of multiple receptor sites for the psychotomimetic agents phencyclidine (PCP) and some opiate-benzomorphans such as (+)N-allylnormetazocine ([+]SKF 10,047) in the mammalian central nervous system is well documented. These are: 1) σ/PCP (σp) site, which binds both PCP and psychotomimetic opiates but not antipsychotics such as haloperidol, 2) PCP site, which selectively binds PCP analogs, and 3) σ/haloperidol (σh) site, for which certain antipsychotics and (+)SKF 10,047, but not PCP analogs, display high affinity. In this study we examined the regulation of these receptor sites after chronic treatment of mice with either PCP or haloperidol. The following radiolabele ligands were used to assess binding to the various receptor subtypes: [3H]-1-[1-[3-hydroxyphenyl)cyclohexyl]piperidine ([3H]PCP-3-OH; σp and PCP sites), [3H]thienyl-phencyclidine ([3H]TCP; PCP site), (+)-[3H]SKF 10,047 (σp and σh sites), and [3H]haloperidol (σh and D-2 dopamine receptors). Treatment of mice for 1, 7, 14, and 21 days with PCP (10 mg·kg-1·day-1) failed to induce variations in σp, σh, and PCP receptor binding. However, similar treatment with haloperidol (4 mg·kg-1·day-1) induced: 1) complete elimination of the binding to σh sites, 2) up-regulation of D-2 dopamine receptors, and 3) no change in σp and PCP receptor binding after 14 or 21 days of treatment. However, a single day of haloperidol treatment or in vitro incubation of mouse brain membranes with haloperidol failed to alter receptor binding. This study suggests that prolonged treatment of mice with haloperidol induces a loss in σh receptors that are presumably associated with certain psychotomimetic effects. This phenomenon is accompanied by an up-regulation of D-2 dopamine receptors.

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