Differential regulation and polyadenylation of transferrin mRNA in Xenopus liver and oviduct

Ricardo Pastori, John E. Moskaitis, Susan W. Buzek, Daniel R. Schoenberg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Estrogen destabilizes transferrin mRNA in male Xenopus liver in the same manner as observed for albumin and γ-fibrinogen. The present study examined estrogen regulation of transferrin gene expression in female Xenopus liver and oviduct. In female Xenopus liver estrogen causes the same enhanced degradation of transferrin mRNA from the cytoplasm as seen in males. In contrast, transferrin is induced 3- to 4-fold in both oviduct nuclear and cytoplasmic RNA. The similar increase in transferrin RNA in both preparations suggests a transcriptional mechanism is responsible for this stimulation. Therefore, transferrin expression is differentially regulated in these tissues by the same hormone. Previous experiments showed that Xenopus serum albumin mRNA has a very short (17 residue) poly(A) tail that may play a role in its hormone-regulated instability. Transferrin mRNA has a similarly short poly(A) tail in liver of both male and female Xenopus. Estrogen has no effect on transferrin polyadenylation in liver. Similarly short poly(A) is found on transferrin mRNA from estrogen-deprived oviducts in explant culture. However, addition of estradiol to the medium results in the appearance of a 50-200 nucleotide poly(A) concurrent with induction. Therefore, transferrin mRNA is differentially polyadenylated in Xenopus liver and oviduct. In the latter tissue polyadenylation is under hormonal control.

Original languageEnglish
Pages (from-to)649-657
Number of pages9
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume42
Issue number7
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Polyadenylation
Oviducts
Transferrin
Xenopus
Liver
Messenger RNA
Estrogens
Poly A
Hormones
RNA
Tissue
Nuclear RNA
RNA Stability
Gene Expression Regulation
Gene expression
Serum Albumin
Fibrinogen
Albumins
Estradiol
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Differential regulation and polyadenylation of transferrin mRNA in Xenopus liver and oviduct. / Pastori, Ricardo; Moskaitis, John E.; Buzek, Susan W.; Schoenberg, Daniel R.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 42, No. 7, 01.01.1992, p. 649-657.

Research output: Contribution to journalArticle

Pastori, Ricardo ; Moskaitis, John E. ; Buzek, Susan W. ; Schoenberg, Daniel R. / Differential regulation and polyadenylation of transferrin mRNA in Xenopus liver and oviduct. In: Journal of Steroid Biochemistry and Molecular Biology. 1992 ; Vol. 42, No. 7. pp. 649-657.
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