Differential protein expression in pancreatic islets after treatment with an imidazoline compound

T. Jägerbrink, H. Lexander, C. Palmberg, J. Shafqat, V. Sharoyko, P. O. Berggren, S. Efendic, S. Zaitsev, H. Jörnvall

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

Original languageEnglish (US)
Pages (from-to)1310-1316
Number of pages7
JournalCellular and Molecular Life Sciences
Volume64
Issue number10
DOIs
StatePublished - May 1 2007

Keywords

  • 2-D gel electrophoresis
  • BL11282
  • Imidazolines
  • Pancreatic islets
  • Proteomics
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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    Jägerbrink, T., Lexander, H., Palmberg, C., Shafqat, J., Sharoyko, V., Berggren, P. O., Efendic, S., Zaitsev, S., & Jörnvall, H. (2007). Differential protein expression in pancreatic islets after treatment with an imidazoline compound. Cellular and Molecular Life Sciences, 64(10), 1310-1316. https://doi.org/10.1007/s00018-007-7136-5