Differential protein expression in pancreatic islets after treatment with an imidazoline compound

T. Jägerbrink, H. Lexander, C. Palmberg, J. Shafqat, V. Sharoyko, P. O. Berggren, S. Efendic, S. Zaitsev, H. Jörnvall

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

Original languageEnglish
Pages (from-to)1310-1316
Number of pages7
JournalCellular and Molecular Life Sciences
Volume64
Issue number10
DOIs
StatePublished - May 1 2007
Externally publishedYes

Fingerprint

Imidazolines
Islets of Langerhans
BL 11282
Rats
Proteins
Annexin A1
Calreticulin
Chaperonin 60
Protein Disulfide-Isomerases
Protein folding
Pyruvate Kinase
Phosphopyruvate Hydratase
Protein C Inhibitor
Protein Folding
Electrophoresis, Gel, Two-Dimensional
Protein Kinase Inhibitors
Medical problems
Cell Extracts
Electrophoresis
Metabolism

Keywords

  • 2-D gel electrophoresis
  • BL11282
  • Imidazolines
  • Pancreatic islets
  • Proteomics
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Jägerbrink, T., Lexander, H., Palmberg, C., Shafqat, J., Sharoyko, V., Berggren, P. O., ... Jörnvall, H. (2007). Differential protein expression in pancreatic islets after treatment with an imidazoline compound. Cellular and Molecular Life Sciences, 64(10), 1310-1316. https://doi.org/10.1007/s00018-007-7136-5

Differential protein expression in pancreatic islets after treatment with an imidazoline compound. / Jägerbrink, T.; Lexander, H.; Palmberg, C.; Shafqat, J.; Sharoyko, V.; Berggren, P. O.; Efendic, S.; Zaitsev, S.; Jörnvall, H.

In: Cellular and Molecular Life Sciences, Vol. 64, No. 10, 01.05.2007, p. 1310-1316.

Research output: Contribution to journalArticle

Jägerbrink, T, Lexander, H, Palmberg, C, Shafqat, J, Sharoyko, V, Berggren, PO, Efendic, S, Zaitsev, S & Jörnvall, H 2007, 'Differential protein expression in pancreatic islets after treatment with an imidazoline compound', Cellular and Molecular Life Sciences, vol. 64, no. 10, pp. 1310-1316. https://doi.org/10.1007/s00018-007-7136-5
Jägerbrink, T. ; Lexander, H. ; Palmberg, C. ; Shafqat, J. ; Sharoyko, V. ; Berggren, P. O. ; Efendic, S. ; Zaitsev, S. ; Jörnvall, H. / Differential protein expression in pancreatic islets after treatment with an imidazoline compound. In: Cellular and Molecular Life Sciences. 2007 ; Vol. 64, No. 10. pp. 1310-1316.
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