Differential production of IL-10 by T cells and monocytes of HIV- infected individuals: Association of IL-10 production with CD28-mediated immune responsiveness

A. Kumar, J. B. Angel, M. P. Daftarian, K. Parato, W. D. Cameron, L. Filion, F. Diaz-Mitoma

Research output: Contribution to journalArticle

31 Scopus citations


Immune unresponsiveness in HIV-1 infection can result from impaired signals delivered by the costimulatory CD28-B7 pathway and the altered production of immunoregulatory cytokines, in particular IL-10, whose production is altered in HIV-1 infection. In this study we investigate IL-10 regulation in T cells and monocytes from HIV+ individuals, and its association with CD28-mediated T cell proliferation. IL-10 production as analysed in T cell- and monocyte-depleted peripheral blood mononuclear cells (PBMC), and by intracellular staining at the single-cell level, reveals a defect in ILl0 production by CD4+ and CD8+ T cells, whereas monocytes constitute the major IL-10-producing cell type. To investigate the impact of IL-10 on immune responsiveness, CD28-mediated proliferative responses in HIV+ individuals were correlated with PHA-induced IL-10 production. CD4+ T cells expressed CD28, yet exhibited markedly reduced CD28-mediated cell proliferation. This CD28-mediated CD4+ T cell proliferation was found to be inversely associated with the levels of PHA-induced IL-10 production and could be restored, at least in part, by anti-IL-10 antibodies. These results suggest that IL-10 production is differentially regulated in T cells and monocytes of HIV+ individuals, and that IL-10 may have a role in inducing immune unresponsiveness by modulating the CD28-B7 pathway.

Original languageEnglish (US)
Pages (from-to)78-86
Number of pages9
JournalClinical and Experimental Immunology
Issue number1
StatePublished - Oct 8 1998



  • CD28
  • HIV-1
  • IL-10

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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