Differential production of IL-10 by T cells and monocytes of HIV- infected individuals

Association of IL-10 production with CD28-mediated immune responsiveness

A. Kumar, J. B. Angel, M. P. Daftarian, K. Parato, W. D. Cameron, L. Filion, F. Diaz-Mitoma

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Immune unresponsiveness in HIV-1 infection can result from impaired signals delivered by the costimulatory CD28-B7 pathway and the altered production of immunoregulatory cytokines, in particular IL-10, whose production is altered in HIV-1 infection. In this study we investigate IL-10 regulation in T cells and monocytes from HIV+ individuals, and its association with CD28-mediated T cell proliferation. IL-10 production as analysed in T cell- and monocyte-depleted peripheral blood mononuclear cells (PBMC), and by intracellular staining at the single-cell level, reveals a defect in ILl0 production by CD4+ and CD8+ T cells, whereas monocytes constitute the major IL-10-producing cell type. To investigate the impact of IL-10 on immune responsiveness, CD28-mediated proliferative responses in HIV+ individuals were correlated with PHA-induced IL-10 production. CD4+ T cells expressed CD28, yet exhibited markedly reduced CD28-mediated cell proliferation. This CD28-mediated CD4+ T cell proliferation was found to be inversely associated with the levels of PHA-induced IL-10 production and could be restored, at least in part, by anti-IL-10 antibodies. These results suggest that IL-10 production is differentially regulated in T cells and monocytes of HIV+ individuals, and that IL-10 may have a role in inducing immune unresponsiveness by modulating the CD28-B7 pathway.

Original languageEnglish (US)
Pages (from-to)78-86
Number of pages9
JournalClinical and Experimental Immunology
Volume114
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

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Interleukin-10
Monocytes
HIV
T-Lymphocytes
Cell Proliferation
HIV Infections
HIV-1
Blood Cells
Staining and Labeling
Cytokines
Antibodies

Keywords

  • CD28
  • HIV-1
  • IL-10

ASJC Scopus subject areas

  • Immunology

Cite this

Differential production of IL-10 by T cells and monocytes of HIV- infected individuals : Association of IL-10 production with CD28-mediated immune responsiveness. / Kumar, A.; Angel, J. B.; Daftarian, M. P.; Parato, K.; Cameron, W. D.; Filion, L.; Diaz-Mitoma, F.

In: Clinical and Experimental Immunology, Vol. 114, No. 1, 1998, p. 78-86.

Research output: Contribution to journalArticle

Kumar, A. ; Angel, J. B. ; Daftarian, M. P. ; Parato, K. ; Cameron, W. D. ; Filion, L. ; Diaz-Mitoma, F. / Differential production of IL-10 by T cells and monocytes of HIV- infected individuals : Association of IL-10 production with CD28-mediated immune responsiveness. In: Clinical and Experimental Immunology. 1998 ; Vol. 114, No. 1. pp. 78-86.
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