Differential inhibitors of DNA polymerases alpha and delta

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Abstract

DNA polymerases alpha and delta from bone marrow are similar in many respects, the major known difference being the exonuclease activity of delta. Differential inhibitors of alpha and delta have been sought to assist in their functional and physical separation. Butylphenyl deoxyguanosine triphosphate is one. It effectively inhibits alpha at less than 1 uM concentration, whereas more than 100 uM is required to similarly inhibit delta. Another is the monoclonal antibody, SJK 132-20, which neutralizes the polymerase activity of alpha but not delta. These differential inhibitors further define alpha and delta as separate categories of eukaryotic DNA polymerase and promise to facilitate the study of both.

Original languageEnglish (US)
Pages (from-to)628-634
Number of pages7
JournalBiochemical and biophysical research communications
Volume132
Issue number2
DOIs
StatePublished - Oct 30 1985

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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