Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in response to heavy metal selection

Paul Odhiambo Mireji, Joseph Keating, Eucharia Kenya, Charles Mbogo, Hudson Nyambaka, Ellie Osir, John Githure, John C Beier

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Investigations were conducted to establish the magnitude and pattern of differential expression of proteins due to generational selection of third instar Anopheles gambiae s.s. Giles larvae by cadmium, copper and lead heavy metals, the three possible common urban pollutants. A susceptible strain of A. gambiae s.s. third instar larvae was separately placed under selection pressure with cadmium, copper and lead at LC30 and controls through five generations. First, third and fifth generation selection survivors were screened for differentially expressed proteins relative to non-exposed control by two-dimensional gel electrophoresis. Distribution patterns of the spots were analyzed by χ2 or Fishers' exact test and variations in expressions between and within generations by ANOVA. Most differentially expressed spots were acidic and of low molecular weight among all metals and generations. Type of heavy metal and generation were the main indicators of variations in differential expressions. Variation between generations was most significant among cadmium-selected populations of which the most number of spots were induced in the fifth generation. Most spots were induced in the copper-selected population in the third generation. The induced protein spots may be the products from respective genes that respond to heavy metals and counter their toxicity, thus building A. gambiae s.s. tolerance to these pollutants. The differential pattern and magnitude of expressed spots have potential application as molecular markers for assessment of anopheline adaptation status to heavy metals, and provide insight into the extent of environmental pollution.

Original languageEnglish
Pages (from-to)214-226
Number of pages13
JournalInternational Journal of Tropical Insect Science
Volume26
Issue number4
DOIs
StatePublished - Dec 1 2006

Fingerprint

Anopheles gambiae
Culicidae
heavy metals
heavy metal
larva
cadmium
protein
larvae
copper
instars
proteins
pollutants
two-dimensional gel electrophoresis
electrokinesis
gel
protein synthesis
analysis of variance
tolerance
pollution
metals

Keywords

  • Adaptation
  • Anopheles gambiae sensu stricto
  • Differential expression
  • Heavy metals
  • Tolerance

ASJC Scopus subject areas

  • Insect Science

Cite this

Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera : Culicidae) larvae in response to heavy metal selection. / Mireji, Paul Odhiambo; Keating, Joseph; Kenya, Eucharia; Mbogo, Charles; Nyambaka, Hudson; Osir, Ellie; Githure, John; Beier, John C.

In: International Journal of Tropical Insect Science, Vol. 26, No. 4, 01.12.2006, p. 214-226.

Research output: Contribution to journalArticle

Mireji, Paul Odhiambo ; Keating, Joseph ; Kenya, Eucharia ; Mbogo, Charles ; Nyambaka, Hudson ; Osir, Ellie ; Githure, John ; Beier, John C. / Differential induction of proteins in Anopheles gambiae sensu stricto (Diptera : Culicidae) larvae in response to heavy metal selection. In: International Journal of Tropical Insect Science. 2006 ; Vol. 26, No. 4. pp. 214-226.
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