Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation

Maria E. Marin-Castaño, Joost P. Schanstra, Françoise Praddaude, João B. Pesquero, Jean Louis Ader, Jean Pierre Girolami, Jean Loup Bascands

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background. Under physiological conditions, the effects of kinins in the kidney are mainly mediated by the bradykinin B2-receptor, whereas the kinin B1-receptor is strongly induced under inflammatory conditions in a variety of tissues. Knowledge of the distribution of the B1-receptor along the nephron is of importance since the B1-receptor might replace B2-receptors under these conditions. Methods. Using a RT-PCR/Southern blot approach allowing relative quantification of mRNA levels, ten different microdissected rat nephron segments were analyzed for the presence of the B1- and B2-receptor before and after endotoxin treatment to induce experimental inflammation. The functionality of the expressed receptors was assessed by kinin-induced intracellular calcium ([Ca2+](i)) mobilization in microdissected nephron segments. Results. While under physiological conditions no B1-receptor mRNA could be detected, after 18 hours of treatment with bacterial lipopolysaccharide (LPS) the expression of B1-receptor mRNA was strongly induced in the efferent arteriole, the medullary and inner medullary thin limb, and in the distal tubule. Moderate expression was found in the glomerulus, proximal convoluted and straight tubules, and in the medullary thick ascending limb. Small but detectable expression was observed in the cortical collecting duct. The induction of B1-receptor mRNA expression resulted in functional receptor expression, since increases in [Ca2+](i) were observed upon B1-agonist stimulation. LPS treatment also increased the expression of B2-receptor mRNA in all nephron segments except in the glomerulus, the inner medullary thin limb and the outer medullary collecting duct. However, no related changes in B2-agonist induced rises in [Ca2+](i) were found. Conclusions. These studies show a functional induction of the B1- kinin receptor along the rat nephron, which should be taken in account to address the effects of kinins under inflammatory conditions in the kidney.

Original languageEnglish
Pages (from-to)1888-1898
Number of pages11
JournalKidney International
Volume54
Issue number6
DOIs
StatePublished - Dec 3 1998
Externally publishedYes

Fingerprint

Bradykinin B1 Receptors
Kinins
Nephrons
Endotoxins
Inflammation
Messenger RNA
Extremities
Lipopolysaccharides
Bradykinin B2 Receptors
Kidney
Arterioles
Southern Blotting
Calcium
Polymerase Chain Reaction

Keywords

  • Des-Argbradykinin
  • Inducible gene expression
  • Kinin-receptors
  • Microdissection
  • Nephron

ASJC Scopus subject areas

  • Nephrology

Cite this

Marin-Castaño, M. E., Schanstra, J. P., Praddaude, F., Pesquero, J. B., Ader, J. L., Girolami, J. P., & Bascands, J. L. (1998). Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation. Kidney International, 54(6), 1888-1898. https://doi.org/10.1046/j.1523-1755.1998.00201.x

Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation. / Marin-Castaño, Maria E.; Schanstra, Joost P.; Praddaude, Françoise; Pesquero, João B.; Ader, Jean Louis; Girolami, Jean Pierre; Bascands, Jean Loup.

In: Kidney International, Vol. 54, No. 6, 03.12.1998, p. 1888-1898.

Research output: Contribution to journalArticle

Marin-Castaño, ME, Schanstra, JP, Praddaude, F, Pesquero, JB, Ader, JL, Girolami, JP & Bascands, JL 1998, 'Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation', Kidney International, vol. 54, no. 6, pp. 1888-1898. https://doi.org/10.1046/j.1523-1755.1998.00201.x
Marin-Castaño, Maria E. ; Schanstra, Joost P. ; Praddaude, Françoise ; Pesquero, João B. ; Ader, Jean Louis ; Girolami, Jean Pierre ; Bascands, Jean Loup. / Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation. In: Kidney International. 1998 ; Vol. 54, No. 6. pp. 1888-1898.
@article{2fcfc463e4e44dd3a577db227d25f151,
title = "Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation",
abstract = "Background. Under physiological conditions, the effects of kinins in the kidney are mainly mediated by the bradykinin B2-receptor, whereas the kinin B1-receptor is strongly induced under inflammatory conditions in a variety of tissues. Knowledge of the distribution of the B1-receptor along the nephron is of importance since the B1-receptor might replace B2-receptors under these conditions. Methods. Using a RT-PCR/Southern blot approach allowing relative quantification of mRNA levels, ten different microdissected rat nephron segments were analyzed for the presence of the B1- and B2-receptor before and after endotoxin treatment to induce experimental inflammation. The functionality of the expressed receptors was assessed by kinin-induced intracellular calcium ([Ca2+](i)) mobilization in microdissected nephron segments. Results. While under physiological conditions no B1-receptor mRNA could be detected, after 18 hours of treatment with bacterial lipopolysaccharide (LPS) the expression of B1-receptor mRNA was strongly induced in the efferent arteriole, the medullary and inner medullary thin limb, and in the distal tubule. Moderate expression was found in the glomerulus, proximal convoluted and straight tubules, and in the medullary thick ascending limb. Small but detectable expression was observed in the cortical collecting duct. The induction of B1-receptor mRNA expression resulted in functional receptor expression, since increases in [Ca2+](i) were observed upon B1-agonist stimulation. LPS treatment also increased the expression of B2-receptor mRNA in all nephron segments except in the glomerulus, the inner medullary thin limb and the outer medullary collecting duct. However, no related changes in B2-agonist induced rises in [Ca2+](i) were found. Conclusions. These studies show a functional induction of the B1- kinin receptor along the rat nephron, which should be taken in account to address the effects of kinins under inflammatory conditions in the kidney.",
keywords = "Des-Argbradykinin, Inducible gene expression, Kinin-receptors, Microdissection, Nephron",
author = "Marin-Casta{\~n}o, {Maria E.} and Schanstra, {Joost P.} and Fran{\cc}oise Praddaude and Pesquero, {Jo{\~a}o B.} and Ader, {Jean Louis} and Girolami, {Jean Pierre} and Bascands, {Jean Loup}",
year = "1998",
month = "12",
day = "3",
doi = "10.1046/j.1523-1755.1998.00201.x",
language = "English",
volume = "54",
pages = "1888--1898",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Differential induction of functional B1-bradykinin receptors along the rat nephron in endotoxin induced inflammation

AU - Marin-Castaño, Maria E.

AU - Schanstra, Joost P.

AU - Praddaude, Françoise

AU - Pesquero, João B.

AU - Ader, Jean Louis

AU - Girolami, Jean Pierre

AU - Bascands, Jean Loup

PY - 1998/12/3

Y1 - 1998/12/3

N2 - Background. Under physiological conditions, the effects of kinins in the kidney are mainly mediated by the bradykinin B2-receptor, whereas the kinin B1-receptor is strongly induced under inflammatory conditions in a variety of tissues. Knowledge of the distribution of the B1-receptor along the nephron is of importance since the B1-receptor might replace B2-receptors under these conditions. Methods. Using a RT-PCR/Southern blot approach allowing relative quantification of mRNA levels, ten different microdissected rat nephron segments were analyzed for the presence of the B1- and B2-receptor before and after endotoxin treatment to induce experimental inflammation. The functionality of the expressed receptors was assessed by kinin-induced intracellular calcium ([Ca2+](i)) mobilization in microdissected nephron segments. Results. While under physiological conditions no B1-receptor mRNA could be detected, after 18 hours of treatment with bacterial lipopolysaccharide (LPS) the expression of B1-receptor mRNA was strongly induced in the efferent arteriole, the medullary and inner medullary thin limb, and in the distal tubule. Moderate expression was found in the glomerulus, proximal convoluted and straight tubules, and in the medullary thick ascending limb. Small but detectable expression was observed in the cortical collecting duct. The induction of B1-receptor mRNA expression resulted in functional receptor expression, since increases in [Ca2+](i) were observed upon B1-agonist stimulation. LPS treatment also increased the expression of B2-receptor mRNA in all nephron segments except in the glomerulus, the inner medullary thin limb and the outer medullary collecting duct. However, no related changes in B2-agonist induced rises in [Ca2+](i) were found. Conclusions. These studies show a functional induction of the B1- kinin receptor along the rat nephron, which should be taken in account to address the effects of kinins under inflammatory conditions in the kidney.

AB - Background. Under physiological conditions, the effects of kinins in the kidney are mainly mediated by the bradykinin B2-receptor, whereas the kinin B1-receptor is strongly induced under inflammatory conditions in a variety of tissues. Knowledge of the distribution of the B1-receptor along the nephron is of importance since the B1-receptor might replace B2-receptors under these conditions. Methods. Using a RT-PCR/Southern blot approach allowing relative quantification of mRNA levels, ten different microdissected rat nephron segments were analyzed for the presence of the B1- and B2-receptor before and after endotoxin treatment to induce experimental inflammation. The functionality of the expressed receptors was assessed by kinin-induced intracellular calcium ([Ca2+](i)) mobilization in microdissected nephron segments. Results. While under physiological conditions no B1-receptor mRNA could be detected, after 18 hours of treatment with bacterial lipopolysaccharide (LPS) the expression of B1-receptor mRNA was strongly induced in the efferent arteriole, the medullary and inner medullary thin limb, and in the distal tubule. Moderate expression was found in the glomerulus, proximal convoluted and straight tubules, and in the medullary thick ascending limb. Small but detectable expression was observed in the cortical collecting duct. The induction of B1-receptor mRNA expression resulted in functional receptor expression, since increases in [Ca2+](i) were observed upon B1-agonist stimulation. LPS treatment also increased the expression of B2-receptor mRNA in all nephron segments except in the glomerulus, the inner medullary thin limb and the outer medullary collecting duct. However, no related changes in B2-agonist induced rises in [Ca2+](i) were found. Conclusions. These studies show a functional induction of the B1- kinin receptor along the rat nephron, which should be taken in account to address the effects of kinins under inflammatory conditions in the kidney.

KW - Des-Argbradykinin

KW - Inducible gene expression

KW - Kinin-receptors

KW - Microdissection

KW - Nephron

UR - http://www.scopus.com/inward/record.url?scp=7844243965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7844243965&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.1998.00201.x

DO - 10.1046/j.1523-1755.1998.00201.x

M3 - Article

VL - 54

SP - 1888

EP - 1898

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 6

ER -