Abstract
The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU-E) and granulocytic (CFU-C) colony forming units have been studied. Both drugs inhibited CFU-E growth in a concentration-dependent, stereospecific, manner. Complete inhibition of human CFU-E growth was observed at a CAP concentration of 10 μg/ml while a concentration over 50 μg/ml was required to inhibit CFU-C growth. Furthermore, whereas the inhibition of CFU-C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU-E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 355-363 |
| Number of pages | 9 |
| Journal | American Journal of Hematology |
| Volume | 2 |
| Issue number | 4 |
| State | Published - 1977 |
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- Hematology
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Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol. / Yunis, Adel A; Adamson, J. W.
In: American Journal of Hematology, Vol. 2, No. 4, 1977, p. 355-363.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol
AU - Yunis, Adel A
AU - Adamson, J. W.
PY - 1977
Y1 - 1977
N2 - The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU-E) and granulocytic (CFU-C) colony forming units have been studied. Both drugs inhibited CFU-E growth in a concentration-dependent, stereospecific, manner. Complete inhibition of human CFU-E growth was observed at a CAP concentration of 10 μg/ml while a concentration over 50 μg/ml was required to inhibit CFU-C growth. Furthermore, whereas the inhibition of CFU-C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU-E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.
AB - The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU-E) and granulocytic (CFU-C) colony forming units have been studied. Both drugs inhibited CFU-E growth in a concentration-dependent, stereospecific, manner. Complete inhibition of human CFU-E growth was observed at a CAP concentration of 10 μg/ml while a concentration over 50 μg/ml was required to inhibit CFU-C growth. Furthermore, whereas the inhibition of CFU-C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU-E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017753705&partnerID=8YFLogxK
M3 - Article
C2 - 602925
AN - SCOPUS:0017753705
VL - 2
SP - 355
EP - 363
JO - American Journal of Hematology
JF - American Journal of Hematology
SN - 0361-8609
IS - 4
ER -