Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol

Adel A. Yunis; John W. Adamson

doi:10.1002/ajh.2830020405

Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol

Adel A. Yunis, John W. Adamson

Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU‐E) and granulocytic (CFU‐C) colony forming units have been studied. Both drugs inhibited CFU‐E growth in a concentration‐dependent, stereospecific, manner. Complete inhibition of human CFU‐E growth was observed at a CAP concentration of 10 μg/ml while a concentration over 50 μ/ml was required to inhibit CFU‐C growth. Furthermore, whereas the inhibition of CFU‐C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU‐E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.

Original language	English (US)
Pages (from-to)	355-363
Number of pages	9
Journal	American Journal of Hematology
Volume	2
Issue number	4
DOIs	https://doi.org/10.1002/ajh.2830020405
State	Published - 1977

Keywords

chloramphenicol
marrow erythroid colonies
thiamphenicol

ASJC Scopus subject areas

Hematology

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title = "Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol",

abstract = "The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU‐E) and granulocytic (CFU‐C) colony forming units have been studied. Both drugs inhibited CFU‐E growth in a concentration‐dependent, stereospecific, manner. Complete inhibition of human CFU‐E growth was observed at a CAP concentration of 10 μg/ml while a concentration over 50 μ/ml was required to inhibit CFU‐C growth. Furthermore, whereas the inhibition of CFU‐C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU‐E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.",

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