Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse

Peter Takacs, Marc Gualtieri, Mehdi Nassiri, Keith Candiotti, Alessia Fornoni, Carlos A. Medina

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Objective: To compare smooth muscle regulatory protein expression in the uterosacral ligament (USL) of women with and without uterine prolapse. Study Design: USLs ligament were sampled in women with (n = 9) or without (n = 9) uterine prolapse. Caldesmon, smooth muscle actin (SMA), myosin heavy chain, and zinc finger protein messenger RNA expression was assessed by quantitative real-time polymerase chain reaction. Immunohistochemistry and digital image analysis were used to determine protein expression. Results: Caldesmon messenger RNA expression and the ratio of caldesmon-SMA messenger RNA expression was significantly increased in the USL from women with uterine prolapse compared with women without prolapse (caldesmon mean ± standard deviation messenger RNA, 0.81 ± 0.46 vs 0.39 ± 0.16; P = .01 and caldesmon-SMA messenger RNA ratio, mean ± standard deviation, 0.11 ± 0.04 vs 0.07 ± 0.02; P = .01). In addition, the ratio of caldesmon-SMA staining was significantly increased in women with uterine prolapse compared with women without prolapse (mean ± standard deviation, 0.44 ± 0.28 vs 0.28 ± 0.16; P = .03). Conclusion: Uterine prolapse is associated with an increased ratio of caldesmon-SMA actin expression.

Original languageEnglish (US)
Pages (from-to)620.e1-620.e5
JournalAmerican journal of obstetrics and gynecology
Volume202
Issue number6
DOIs
StatePublished - Jun 2010

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Keywords

  • caldesmon
  • prolapse
  • smooth muscle cell
  • uterosacral ligament

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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