Differential expression of S1 and elongation factor-1α during rat development

Stephen Lee, L. A. Wolfraim, E. Wang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Elongation factor-1α (EF-1α) is a highly conserved protein functioning in peptide elongation during translation. A cDNA, S1, was isolated; its deduced amino acid sequence shares high similarity with mammalian EF-1αs (92%). While EF-1α is present in all tissues, S1 mRNA can only be detected in brain, heart, and muscle. We report here that the retropseudogene phenomenon is attributable to EF-1α and not S1, the latter being represented by a single copy in the rat genome. The S1 steady-state mRNA levels are consistently higher than EF-1α in S1-positive tissues. S1 mRNA can only be detected late during brain, heart, and muscle development in vivo and increases to a plateau in early postnatal life. In a cultured muscle system, S1 expression is dependent upon the formation of myotubes, although the accumulation of S1 mRNA is significantly lower than that observed in adult skeletal muscle. EF-1α mRNA levels are down-regulated during brain, heart, and muscle development, but stay relatively steady in liver. We show here that EF-1α and S1 are differentially expressed during rat development and that the activation of S1 gene expression is subsequent to the terminal differentiation process in brain, heart, and muscle.

Original languageEnglish (US)
Pages (from-to)24453-24459
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number32
StatePublished - 1993
Externally publishedYes

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Peptide Elongation Factor 1
Muscle
Rats
Brain
Myocardium
Messenger RNA
Muscle Development
Tissue
Skeletal Muscle Fibers
Gene expression
Liver
Elongation
Amino Acid Sequence
Skeletal Muscle
Complementary DNA
Genes
Chemical activation
Genome
Gene Expression
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential expression of S1 and elongation factor-1α during rat development. / Lee, Stephen; Wolfraim, L. A.; Wang, E.

In: Journal of Biological Chemistry, Vol. 268, No. 32, 1993, p. 24453-24459.

Research output: Contribution to journalArticle

Lee, Stephen ; Wolfraim, L. A. ; Wang, E. / Differential expression of S1 and elongation factor-1α during rat development. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 32. pp. 24453-24459.
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