Elongation factor-1α (EF-1α) is a highly conserved protein functioning in peptide elongation during translation. A cDNA, S1, was isolated; its deduced amino acid sequence shares high similarity with mammalian EF-1αs (92%). While EF-1α is present in all tissues, S1 mRNA can only be detected in brain, heart, and muscle. We report here that the retropseudogene phenomenon is attributable to EF-1α and not S1, the latter being represented by a single copy in the rat genome. The S1 steady-state mRNA levels are consistently higher than EF-1α in S1-positive tissues. S1 mRNA can only be detected late during brain, heart, and muscle development in vivo and increases to a plateau in early postnatal life. In a cultured muscle system, S1 expression is dependent upon the formation of myotubes, although the accumulation of S1 mRNA is significantly lower than that observed in adult skeletal muscle. EF-1α mRNA levels are down-regulated during brain, heart, and muscle development, but stay relatively steady in liver. We show here that EF-1α and S1 are differentially expressed during rat development and that the activation of S1 gene expression is subsequent to the terminal differentiation process in brain, heart, and muscle.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Biological Chemistry|
|State||Published - Jan 1 1993|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology