Differential expression of miRNA-146a-regulated inflammatory genes in human primary neural, astroglial and microglial cells

Yuan Yuan Li, Jian Guo Cui, Prerna Dua, Aileen I. Pogue, Surjyadipta Bhattacharjee, Walter J. Lukiw

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Micro RNA-146a (miRNA-146a) is an inducible, 22 nucleotide, small RNA over-expressed in Alzheimer's disease (AD) brain. Up-regulated miRNA-146a targets several inflammation-related and membrane-associated messenger RNAs (mRNAs), including those encoding complement factor-H (CFH) and the interleukin-1 receptor associated kinase-1 (IRAK-1), resulting in significant decreases in their expression (p<0.05, ANOVA). In this study we assayed miRNA-146a, CFH, IRAK-1 and tetraspanin-12 (TSPAN12), abundances in primary human neuronal-glial (HNG) co-cultures, in human astroglial (HAG) and microglial (HMG) cells stressed with Aβ42 peptide and tumor necrosis factor alpha (TNFα). The results indicate a consistent inverse relationship between miRNA-146a and CFH, IRAK-1 and TSPAN12 expression levels, and indicate that HNG, HAG and HMG cell types each respond differently to Aβ42-peptide. +. TNFα-triggered stress. While the strongest miRNA-146a-IRAK-1 response was found in HAG cells, the largest miRNA-146a-TSPAN12 response was found in HNG cells, and the most significant miRNA-146a-CFH changes were found in HMG cells, the 'resident scavenging macrophages' of the brain.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalNeuroscience Letters
Volume499
Issue number2
DOIs
StatePublished - Jul 20 2011

Keywords

  • Brain gene expression
  • Complement factor H (CFH)
  • Human astroglial cells
  • Interleukin-1 receptor associated kinase 1 (IRAK-1)
  • Microglial cells
  • MicroRNA (miRNA)
  • Neurodegeneration
  • Neuroinflammation
  • Post-transcriptional control
  • Small RNA
  • Tetraspanin 12 (TSPAN12)

ASJC Scopus subject areas

  • Neuroscience(all)

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