TY - JOUR
T1 - Differential Expression of MicroRNAs in Embryos and Larvae of Mahi-Mahi (Coryphaena hippurus) Exposed to Deepwater Horizon Oil
AU - Diamante, Graciel
AU - Xu, Elvis Genbo
AU - Chen, Shuai
AU - Mager, Edward
AU - Grosell, Martin
AU - Schlenk, Daniel
N1 - Funding Information:
This research was made possible by a grant from The Gulf of Mexico Research Initiative [Grant SA-1520, Relationship of Effects of Cardiac Outcomes in fish for Validation of Ecological Risk (RECOVER)]. M.G. holds a Maytag Chair of Ichthyology. Data are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at https://data.gulfresearchinitiative.org (DOI: 10.7266/ N70Z71QJ).
PY - 2017/12/12
Y1 - 2017/12/12
N2 - Deepwater Horizon (DWH) oil causes developmental cardiotoxicity in fish species, but the molecular mechanisms are still not well understood. MicroRNAs (miRNAs) play key roles in a number of biological processes, including heart development. Therefore, the expression of miRNAs in mahi-mahi (Coryphaena hippurus) exposed to weathered slick oil and nonweathered source oil was analyzed. miRNAs were sequenced and annotated using the Fugu rubripes genome (termed the Fugu-guided approach) or aligned with known mature animal miRNAs using the Basic Local Alignment Search Tool (BLAST) method (termed the phylogenetically guided approach). Exposure of embryos to slick oil resulted in more differentially expressed miRNAs than exposure to source oil at all developmental stages. Gene ontology analysis on the target mRNAs was consistent with pathway analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure, and specific miRNA-mRNA interactions identified by ingenuity pathway analysis. Slick oil caused an overexpression of miR-133, which correlated with the decrease in the level of expression of genes related to the cardiovascular system such as potassium voltage-gated channel subfamily H member 2 (kchn2). This work is the first study linking miRNAs and mRNAs in crude oil-exposed fish, providing a new understanding of the molecular mechanism(s) of oil toxicity.
AB - Deepwater Horizon (DWH) oil causes developmental cardiotoxicity in fish species, but the molecular mechanisms are still not well understood. MicroRNAs (miRNAs) play key roles in a number of biological processes, including heart development. Therefore, the expression of miRNAs in mahi-mahi (Coryphaena hippurus) exposed to weathered slick oil and nonweathered source oil was analyzed. miRNAs were sequenced and annotated using the Fugu rubripes genome (termed the Fugu-guided approach) or aligned with known mature animal miRNAs using the Basic Local Alignment Search Tool (BLAST) method (termed the phylogenetically guided approach). Exposure of embryos to slick oil resulted in more differentially expressed miRNAs than exposure to source oil at all developmental stages. Gene ontology analysis on the target mRNAs was consistent with pathway analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure, and specific miRNA-mRNA interactions identified by ingenuity pathway analysis. Slick oil caused an overexpression of miR-133, which correlated with the decrease in the level of expression of genes related to the cardiovascular system such as potassium voltage-gated channel subfamily H member 2 (kchn2). This work is the first study linking miRNAs and mRNAs in crude oil-exposed fish, providing a new understanding of the molecular mechanism(s) of oil toxicity.
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U2 - 10.1021/acs.estlett.7b00484
DO - 10.1021/acs.estlett.7b00484
M3 - Article
AN - SCOPUS:85038208203
VL - 4
SP - 523
EP - 529
JO - Environmental Science and Technology Letters
JF - Environmental Science and Technology Letters
SN - 2328-8930
IS - 12
ER -