Differential expression of microRNAs among cell populations in the regenerating adult mouse olfactory epithelium

Sarah Kurtenbach, Wen Ding, Garrett M. Goss, Joshua Hare, Bradley J Goldstein, Lina Shehadeh

Research output: Contribution to journalArticle

Abstract

Despite a robust capacity for adult neurogenesis in the olfactory epithelium (OE), olfactory sensory losses are common. Identification of mechanisms regulating adult OE neurogenesis is, therefore, of interest. MicroRNAs (miRNAs) are broadly important in regulating vertebrate neurodevelopment, and are required in embryonic olfactory differentiation. We report here that a panel of miRNAs is differentially expressed by either progenitor or progeny cells in the regenerating mouse OE. Progenitor cells were purified from lesioned OE based on c-Kit expression, and miRNA expression was assayed in c-Kit (+) and c-Kit (-) cell populations. 28 miRNAs were significantly downregulated by at least 4 fold in the c-Kit (+) fraction, which marks the globose basal progenitor cell population. In addition, 10 miRNAs were upregulated in these basal cells. MiR-486, the most strongly downregulated miRNA identified, was further characterized to verify results. MiR-486 expression was confirmed in the c-Kit (-) OE layers using in situ hybridization. As a functional assay, over-expression of miR-486 in purified c-Kit (+) basal cell cultures resulted in a reduction in neurogenesis, consistent with a possible negative feedback regulatory model. Our data provide new insights regarding miRNA expression and function during adult OE neurogenesis, and identify candidate miRNAs warranting further study.

Original languageEnglish (US)
Article numbere0187576
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

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Olfactory Mucosa
MicroRNAs
microRNA
epithelium
Cells
neurogenesis
Neurogenesis
mice
Population
cells
stem cells
Stem Cells
Down-Regulation
neurodevelopment
Cell culture
in situ hybridization
In Situ Hybridization
Vertebrates
Assays
cell culture

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Differential expression of microRNAs among cell populations in the regenerating adult mouse olfactory epithelium. / Kurtenbach, Sarah; Ding, Wen; Goss, Garrett M.; Hare, Joshua; Goldstein, Bradley J; Shehadeh, Lina.

In: PLoS One, Vol. 12, No. 11, e0187576, 01.11.2017.

Research output: Contribution to journalArticle

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