Differential expression of fibulins in the uterosacral ligaments of women with uterine prolapse

Peter Takacs, Mehdi Nassiri, Keith Candiotti, Jinfeng Yang, Sujata Yavagal, Carlos A. Medina

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Purpose: To compare fibulin-3 (FIB-3) and fibulin-5 (FIB-5) expressions in uterosacral ligaments (USL) of women with and without uterine prolapse. Study design: USL were sampled in a standardized fashion from women with (n = 8) or without (n = 8) uterine prolapse. Quantitative Real-Time PCR was performed to measure mRNA and immunohistochemistry to assess protein expression. Results: FIB-3 mRNA expression and FIB-3 staining intensity was similar in the USL of women with and without uterine prolapse {[(FIB-3 mean ± SD mRNA relative units) 0.45 ± 0.41 vs. 0.46 ± 0.82, NS] and [Intensity score, median (range), 1(0-1) vs. 1(0-1), NS]}. Both FIB-5 mRNA expression and FIB-5 staining intensity was significantly decreased in USL from women with uterine prolapse compared to women without prolapse {[(FIB-5 mean ± SD mRNA relative units) 0.07 ± 0.02 vs. 0.26 ± 0.20, P = 0.02] and [Intensity score, median (range), 0(0-2) vs. 3(2-3), P = 0.002]}. Conclusion: FIB-5 expression is decreased in USL of women with uterine prolapse.

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalArchives of gynecology and obstetrics
Volume282
Issue number4
DOIs
StatePublished - Oct 1 2010

Keywords

  • Fibulin
  • Prolapse
  • Uterosacral ligament

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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