Differential effects of opioids on the proliferation of a macrophage cell line, Bac 1.2F5

Sabita Roy, Mohamed Sedqi, Sundaram Ramakrishnan, Roderick A. Barke, Horace H. Loh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Chronic treatment of mice with morphine selectively abrogates the terminal differentiation of com mitted bone marrow progenitor cells to form macrophage colony forming units. To understand the molecular mechanisms involved in morphine-mediated suppression of myeloid cell differentiation, we investigated the use of a macrophage cell line, Bac 1.2F5. In vitro proliferation of this cell line is dependent on the exogenous supply of macrophage colony stimulating factor. Treatment of Bac 1.2FS cells in vitro with morphine showed a dose-dependent inhibition of proliferation which was associated with morphological changes. Characterization of the binding site revealed that the binding site for morphine on these cells is different from the classical opioid receptors described in the brain. In addition to the putative novel class of morphine receptors, Bac 1.2F5 cells also expressed the delta opioid receptors as determined by RT-PCR analyses. These studies show that Bac 1.2F5 cells are suitable for the molecular characterization of opioid effects on the proliferation and differentiation of myeloid progenitor cells.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalCellular Immunology
Volume169
Issue number2
DOIs
StatePublished - May 1 1996
Externally publishedYes

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Morphine
Opioid Analgesics
Macrophages
Cell Line
Stem Cells
Binding Sites
Myeloid Progenitor Cells
delta Opioid Receptor
Macrophage Colony-Stimulating Factor
mu Opioid Receptor
Opioid Receptors
Myeloid Cells
Bone Marrow Cells
Cell Differentiation
Polymerase Chain Reaction
Brain
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Differential effects of opioids on the proliferation of a macrophage cell line, Bac 1.2F5. / Roy, Sabita; Sedqi, Mohamed; Ramakrishnan, Sundaram; Barke, Roderick A.; Loh, Horace H.

In: Cellular Immunology, Vol. 169, No. 2, 01.05.1996, p. 271-277.

Research output: Contribution to journalArticle

Roy, Sabita ; Sedqi, Mohamed ; Ramakrishnan, Sundaram ; Barke, Roderick A. ; Loh, Horace H. / Differential effects of opioids on the proliferation of a macrophage cell line, Bac 1.2F5. In: Cellular Immunology. 1996 ; Vol. 169, No. 2. pp. 271-277.
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